一株拮抗细菌HJ5高产抗菌物质发酵条件优化  

The optimal fermentation conditions of HJ5 for high production of antimicrobial substance

作　　者：辛媛媛[1] 侯美玲[1] 郝志敏[1] 董金皋[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071000

出　　处：《河北农业大学学报》2012年第4期70-74,共5页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2008000335)

摘　　要：从黄顶菊茎中分离得到的内生细菌HJ5,是一株具有拮抗玉米大斑病菌活性的枯草芽孢杆菌。研究发现,HJ5菌株通过产生并分泌某种胞外物质发挥拮抗活性。为了增强HJ5菌株发酵产拮抗物质的能力,对HJ5菌株发酵条件进行单因子试验以及正交试验,从而确定了HJ5菌株发酵产抗菌活性物质的最佳培养基配方为1%葡萄糖作为碳源、1%大豆蛋白胨作为氮源、0.01%MnCl2作为添加无机盐,初始pH为8。对HJ5菌株发酵工艺参数优化结果为:50/250mL的装液量、2%的接种量、28℃180r/min振荡培养66h。经发酵条件优化后较基础培养基,HJ5菌株拮抗活性增强了近1倍。Setosphaeria turcica was inhibited by HJ5 which was an endophytic bacterium isolated from the stem of Flaveria bidentis. The optimum medium were 1% of glucose, 1 % of soy peptone, 0.01% of MnCl2 and primary pH was 8.0 via single factor tests and orthogonal test. The rotating speed of rocking incubator was 180 r/min. The inoeulum and liquid volume in flask were 2% and 50 mL/250 mL, respectively. The cultivating temperature was 28℃through optimizing the fermentation technological parameter. After the optimization, antagonistic activity of HJ5 enhances nearly doubled.

关 键 词：玉米大斑病菌 内生细菌 拮抗 发酵条件 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]