儿茶酚抑制藻细胞活性过程中IOM的三维荧光特性  

Fluorescence Characterization of IOM from Inhibitory Effect on Algal Activity by CAT

作　　者：汪丽[1] 梁文艳[1] 阮铃铃[1] 曹金灿[1] 于建[1] 

机构地区：[1]北京林业大学环境科学与工程学院,北京100083

出　　处：《环境科学与技术》2012年第8期1-5,共5页Environmental Science & Technology

基　　金：国家自然科学基金项目(50678024);国家水体污染控制与治理科技重大专项(2008ZX07314-006)

摘　　要：以天然水体中藻类为研究对象,研究了儿茶酚对藻类的抑制效应,并采用三维荧光法分析了抑藻过程中胞内有机物的释放及降解情况。水样A和水样B分别采自某护城河和一景观水体。结果显示,儿茶酚能够有效抑制实际水体中藻细胞活性,当儿茶酚终浓度为10 mg/L培养6 d后,水样A和水样B的抑藻率分别达90%和94%,且抑藻率随着投加浓度的增加、培养时间的延长相应增加。在投加儿茶酚后,水样类蛋白荧光峰强度显著增加,但随着培养时间的延长,峰强均有所降低,细胞释放的类蛋白物质发生降解。当投加儿茶酚浓度低于1 mg/L时,藻细胞活性受轻度抑制,会释放类富里酸物质,当儿茶酚投加浓度高于5 mg/L,藻细胞活性受到强烈抑制,不会释放类富里酸物质。投加儿茶酚会增大水样COD浓度,但对水样TN浓度没有影响。Inhibitory effect of catechol （CAT）on algal activity from natural waters as well as the release and degradation of intracellular organic matters（ IOM ）resulting from algal inhibition by three--dimensional fluorescence spectroscopy were studied. The algae A and B were sampled from a city moat and a landscape water respectively. Results showed that CAT has significant inhibitory effect on activity of algae, which could reach 90% for sample A and 94% for sample B after 6d at final concentration of CAT 10 mg/L. During the culture period, inhibition ratio increased along with CAT concentration and culture time. Just after adding of CAT, the protein-like fluorescence peak intensity of algal samples increased significantly, but gradually declined over the culture time. Protein-like fluorescent matters released from algal cells could be easily degraded. When concentration of CAT in water was less than 1 mg/L, algal activity was slightly inhibited and then algal cells released fulvic-like substances. While the CAT was greater than 5 mg/L, it caused strongly inhibition effect, and algal cells would not release fulvic-like substances. Addition of CAT would result in the increase of COD concentration, but the concentration of TN was not affected.

关 键 词：儿茶酚 藻类 抑制效应 三维荧光 胞内有机物 

分 类 号：X132[环境科学与工程—环境科学]