重组人促红细胞生成素对脑缺血再灌注小鼠海马IL-1β表达的影响  

Effect of recombinant human erythropoietin on the expression of IL-β in cerebral ischemia-reperfusion mouse

作　　者：王勇[1] 夏俊哲[1] 铁欣昕[1] 姚长义[1] 王运杰[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《解剖学研究》2012年第4期260-263,共4页Anatomy Research

摘　　要：目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对脑缺血再灌注小鼠白介素-1β(interleukin-1β,IL-1β)表达的影响。方法 96只昆明小鼠随机分为3组,每组32只:假手术组(Sham);rHuEPO治疗组;缺血再灌注组(I/R)。每组依据缺血后再灌注不同时间点再分6 h、24 h、3 d、7 d 5个小组。免疫组化和Western blot检测各组小鼠IL-1β的表达。TUNEL法检测海马细胞凋亡情况。结果缺血再灌注组IL-1β蛋白表达量和凋亡细胞数明显高于假手术组(P<0.05),而rhEPO治疗组IL-1β蛋白表达量和凋亡细胞数则明显少于缺血再灌注组(P<0.05)。结论 rHuEPO很可通过下调IL-1β的表达参与脑缺血再灌注损伤。Objective To study the expression of intedeukin-1 β （IL-β） in cerebral ischemia-reperfusion mice after recom- binant human erythropoietin （rhEPO） was injected. Methods 96 Kunming mice were randomly divided into the sham group, ischemia-repeffusion group and recombinant human erythropoietin group at different time points （6 h, 24 h, 3 d and 7 d） on average. The expression of IL-β in hippocampus was examined by immunochemistry and western blot methods. The apoptotic hippocampus cells were stained by TUNEL. Result Both the expression of IL-β and the apoptosis ceils increased significantly in I/R group than in sham group （P〈0.05）. Howerer the expression of IL-β,and the apoptosis cells decreased in recombinant human erythropoietin group compared with I/R group （P〈0.05）. Conclusion one of the mechanisms for recombinant human erythropoietin participate the pathogenesis of cerebral ischemia-repeffusion injury might be downregulate the expression of IL-β.

关 键 词：缺血再灌注 重组人促红细胞生成素 白介素-1Β 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]