儿童胃黏膜白细胞介素mRNA水平与幽门螺旋杆菌感染的研究  

Relationship between gastric mucosal IL-1β mRNA level and H.pylori infection in children with digestive tract diseases

作　　者：李娟[1] 嘉红云[1] 陈浩宇[1] 周强[1] 吴晓蔓[1] 王方金[2] 何蕴韶[2] 

机构地区：[1]广州医学院第二附属医院检验科,510260 [2]中山大学达安基因诊断中心,广东510260

出　　处：《检验医学与临床》2012年第14期1699-1700,共2页Laboratory Medicine and Clinic

摘　　要：目的分析儿童胃黏膜白细胞介素(IL-1β)水平与幽门螺旋杆菌(H.pylori)及其高毒力亚型感染间的相关性。方法实时定量聚合酶链(real-time PCR)检测H.pylori的保守基因ure以判断H.pylori的感染,高毒力亚型基因vacA s1用real-time PCR检测,用PCR扩增另一高毒力基因cagA羧基端EPIYA基序所在区,然后测序确定其亚型。IL-1βmRNA使用PCR-荧光探针法检测,比较循环CT法分析。结果患儿中无论是低毒力还是高毒力的H.pylori感染都与胃黏膜IL-1βmRNA水平无显著相关性。结论 H.pylori对儿童胃黏膜IL-1β表达的影响作用并不明显,可能还有其他一些环境、遗传因素如IL-1基因簇多态性与H.pylori共同作用调节胃黏膜IL-1β的表达。Objective To investigate the gastric mucosal IL-1β level in children and to study its relationship with Helicobacter（H.） pylori and its genotypes.Methods The real-time PCR was adopted to detect the conserved gene ureA to diagnose H.pylori infection.High virulence gene vacA s1 was tested by real-time PCR.EPIYA motifs in the 3′ region of cagA were detected by conventional PCR and DNA sequencing.IL-1β mRNA level was tested by the real-time PCR-fluorescent probe assay and analyzed by CT method.Results In children with peptic symptoms,no matter H.pylori with high or low toxicity,neither of them had relationship with gastric mucosal IL-1β mRNA level.Conclusion H.pylori infection has no evident effect on gastric mucosal IL-1β mRNA production in children.There may be some other environment and genetic factors such as IL-1 gene polymorphisms combined H.pylori infection co-regulating the gastric mucosal IL-1β expression.

关 键 词：白细胞介素 幽门螺旋杆菌 实时荧光定量聚合酶链检测 

分 类 号：R573[医药卫生—消化系统]