肝细胞癌组织中ZEB-1的表达及意义  

Expression of ZEB - 1 in hepatocellular carcinoma tissues and its significance

作　　者：杨晓宇[1] 范飞[2] 付晓辉[1] 李爱军[1] 

机构地区：[1]第二军医大学东方肝胆外科医院特需治疗二科,上海200438 [2]第二军医大学东方肝胆外科医院胆道二科,上海200438

出　　处：《第二军医大学学报》2012年第8期842-845,共4页Academic Journal of Second Military Medical University

基　　金：国家科技重大专项(2008ZX10002-025)~~

摘　　要：目的探讨ZEB-1在肝细胞癌(HCC)组织中的表达及意义。方法采用免疫组织化学SP法和蛋白质印迹分析法检测ZEB-1在62例HCC组织、48例相应癌旁肝硬化组织和10例正常肝组织中的表达,并分析其与HCC临床病理特征的关系。结果免疫组化结果显示ZEB-1在HCC组织及癌旁肝硬化组织中呈阳性表达,阳性率分别为93.5%(58/62)和83.3(40/48)。蛋白质印迹分析显示ZEB-1蛋白在HCC组织中的表达高于相应癌旁肝硬化组织和正常肝组织(P<0.05)。ZEB-1在HCC组织中的表达与临床分期、肿瘤分化程度、有无发生转移、有无门脉癌栓及有无术后复发等因素相关(P<0.05),与肿瘤直径、肿瘤数目、血清AFP水平等因素无关(P>0.05)。结论 ZEB-1的高表达与肝癌的发生、发展密切相关,并且有可能参与HCC细胞的上皮间质转化而促进肝癌的侵袭和转移。Objective To investigate ZEB-1 protein expression in hepatocellular carcinoma（HCC） tissues and the relevant significance.Methods Immunohistochemical method and Western blotting analysis were used to detect the ZEB-1 protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues.The relationship between ZEB-1 protein expression and clinicopathological features of HCC was evaluated.Results Immunohistochemical staining showed that the positive rates of ZEB 1 protein expression in HCC tissues and paracancerous cirrhotic tissues were 93.5%（58/62） and 83.3（40/48）,respectively.Western blotting analysis showed that ZEB-1 protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues（P0.05）.Conclusion High expression of ZEB 1 is correlated with the development and progression of HCC.ZEB-1 might also participate in the epithelial-mesenchymal transition of HCC cells,subsequently contributing to the invasion and metastasis.

关 键 词：肝肿瘤 肝细胞癌 ZEB-1 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]