金丝桃素体外抗乙型肝炎病毒的作用  

The effects of hypericin on inhibition of hepatitis B virus replication in vitro

作　　者：李岩[1] 汤敏[1] 安瑛[1] 寻琳婷[1] 

机构地区：[1]云南省第一人民医院消化内科,昆明650032

出　　处：《中华传染病杂志》2012年第8期459-462,共4页Chinese Journal of Infectious Diseases

摘　　要：目的研究金丝桃素对HBV复制的影响。方法以拉米夫定为对照，浓度梯度金丝桃素作用于HepG2．2．15细胞，ELISA法检测细胞培养上清液中HBsAg和HBeAg，Southern印迹法检测细胞培养上清液中HBVDNA水平，并计算金丝桃素的半数细胞抑制浓度（IC50）和半数有效浓度（EC50）。以32P标记的脱氧核糖核苷三磷酸为底物，测定金丝桃素和拉米夫定对HBVDNA聚合酶（反转录酶）活性。采用独立样本t检验和单因素方差分析进行两组及多组数据间比较。结果随金丝桃素浓度的升高，对HBsAg和HBeAg的抑制率也逐步提高，且〉0．5μmol／L各组抑制率均高于拉米夫定，差异有统计学意义（t=-0．127，P〈0．05）。金丝桃素具有比拉米夫定更强的抑制HBVDNA作用，EC50为0．2μmol／L，差异有统计学意义（t=-0．058，P〈0．05）。EC50（0．2μmol／L）及ICS0（200μmot／L）结果显示，在实验范围内金丝桃素对HepG2．2．15无毒性作用。与拉米夫定不同，金丝桃素对DNA聚合酶（反转录酶）无影响。结论金丝桃素能有效抑制HBV复制及抗原合成，在实验范围内对HepG2．2．15无明显毒性作用，且与传统核苷类化合物具有不同的抗HBV靶点。Objective To explore the effects of hypericin on inhibition of hepatitis B virus （HBV） replication. Methods The concentration gradient hypericin was added to HepG2. 2. 15 cell culture system and lamivudine was used as control. Enzyme-linked immunosorbent assay （ELISA） and Southern blot were used to examine HBsAg, HBeAg and HBV DNA level in the culture supernatant, respectively. Half inhibitory concentration （IC50） and half effective concentration （EC50） of hypericin were calculated. The effects of hypericin on HBV DNA polymerase were detehted by a2p marked deoxy-ribonucleoside triphosphate as the substrate. Independent sample t-test and single factor analysis of variance were used to compare the data between two groups and among multiple groups. Results The inhibition rates of hypericin on HBsAg, HBeAg were enhanced with hypericin concentration increasing and those were higher than lamivudine control group when the concentration was higher than 0.5 μmol/L （t=--0. 127, P〈0.05）. Southern blot confirmed that hypericin was stronger in inhibition of HBV DNA （ECS0=0.2 μmol/L） than lamivudine （t=0. 058, P%0.05）. Hypericin was non-toxic on HepG2.2.15 ceils in the range of test with EC50 of 0.2μmol/L and ICS0 of 200μmol/L. Hyperiein did not act on HBV DNA polymerase which was quite different from lamivudine. Conclusions Hypericin can effectively inhibit HBV replication as well as antigen synthesis and is non-toxic on HepG2.2.15 cell. The anti-HBV target of hypericin is different from nucleos（t）ide analogues.

关 键 词：DNA 病毒 金丝桃素 肝炎病毒 乙型 细胞 培养的 

分 类 号：R[医药卫生]