针刺“足三里”对哮喘大鼠嗜酸细胞凋亡及相关基因表达的影响  

Effects of acupuncture at “Zusanli”(ST 36) on eosinophil apoptosis and related gene expression in rats with asthma

作　　者：吴兆利[1] 李春日[1] 刘自力[2] 张庆荣[3] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847 [2]辽宁中医药大学杏林学院,沈阳110847 [3]辽宁中医药大学基础医学院,沈阳110847

出　　处：《中国针灸》2012年第8期721-725,共5页Chinese Acupuncture & Moxibustion

摘　　要：目的:观察针刺"足三里"对脾虚哮喘和哮喘大鼠的效应差异,并探讨其作用机制。方法:60只SD大鼠按体质量随机分为5组:脾虚哮喘组、脾虚哮喘针刺组、哮喘组、哮喘针刺组、正常对照组,每组12只。前两组先建立脾虚模型,之后与哮喘组及哮喘针刺组一起均采用卵蛋白诱发哮喘动物造模方法,建立大鼠脾虚哮喘结合模型和哮喘模型,正常对照组以同等剂量的生理盐水进行处理。脾虚哮喘针刺组和哮喘针刺组均针刺"足三里"穴进行治疗,每日1次,连续治疗8d,其他组不予治疗干预。采用原位杂交法检测肺组织中Fas基因(mRNA)、Bcl-2mRNA的表达。以脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL)进行肺组织细胞凋亡的检测。结果:与正常对照组比较,脾虚哮喘组和哮喘组均显示为Fas mRNA表达明显减少和Bcl-2mR-NA表达增多(均P<0.01)、嗜酸细胞(EOS)计数显著增多和EOS凋亡率下降(均P<0.01);与哮喘组比较,脾虚哮喘组的Fas mRNA表达明显减少、Bcl-2mRNA表达增多(均P<0.01)、EOS计数明显增多(P<0.01);与两对应的哮喘组比较,两针刺组的Fas mRNA表达明显增多和Bcl-2mRNA表达显著减少(均P<0.01)、EOS计数显著减少和EOS凋亡率明显升高(均P<0.01);哮喘针刺组与脾虚哮喘针刺组比较,Fas mRNA和Bcl-2mRNA的表达无明显差异(均P>0.05),哮喘针刺组的EOS计数显著下降、EOS凋亡率显著提高(均P<0.01)。结论:针刺"足三里"均可调整脾虚哮喘和哮喘状态下Fas mRNA和Bcl-2mRNA表达的失衡状态,促进EOS的凋亡,从而抑制哮喘炎性反应的进一步发展;针刺"足三里"在调整脾虚哮喘EOS相关基因表达失衡上具有一定的优势。Objective To observed efficacy differences of acupuncture at＂Zusanli＂（ST 36）in rats with asthma and asthma with spleen-deficency,so as to investigate the therapeutic mechanism.Methods Sixty SD rats were randomly divided into 5groups according to their weight,named as an asthma with spleen-deficency group（group A）,an acupuncture on asthma with spleen-deficency group（group B）,an asthma group（group C）,an acupunc-ture on asthma group（group D）and a control group.The rat models with spleen-deficiency in the first two groups were set up by TCM,then the rats of asthma model in the first four groups were induced by egg albumin,but the control group was treated by the same dose of saline.The group B and the group D were both treated with acu-puncture at＂Zusanli＂（ST 36）,once each day for 8days,and the other groups remained unhandled.The mRNA expressions of Fas and Bcl-2in the lung tissues were detected by hybridization in situ and apoptosis was detected by TUNEL（terminal dexynucleotidyl transferase-mediated dutp nick end labeling）.Results Compared with the con-trol group,in both the group A and the group C,the expression of Fas mRNA significantly decreased,but the ex-pression of Bcl-2mRNA significantly increased（all P0.05）,but compared with group B,in the group D,EOS counts significantly decreased and EOS apoptosis rate significantly increased（both P〈0.01）.Conclusion Acupuncture at ＂Zusanli＂（ST 36）canregulate the disorders of Fas mRNA and Bcl-2mRNA expression in the state of both asthma and asthma with spleen-deficency,promote EOS apoptosis so as to inhibit the development of inflammatory reaction of asthma,showing that acupuncture at＂Zusanli＂（ST 36）has certain advantages on regulation of related gene of EOS in asthma with spleen-deficency.

关 键 词：哮喘 脾虚 针刺 穴 足三里 FASMRNA BCL-2MRNA 

分 类 号：R246.1[医药卫生—针灸推拿学]