石斛合剂对糖尿病模型大鼠胰岛细胞凋亡及凋亡相关基因Bax、Bcl-2 mRNA表达的影响  

Effect of Dendrobium Compound on Apoptosis and mRNA Expression of Bax and Bcl-2 in Islet Cells in Diabetes Rats

作　　者：高尤亮[1] 施红[2] 杨秀珍[3] 余文珍[2] 郑燕芳[2] 王晓宁[2] 江敏[1] 

机构地区：[1]福建中医药大学附属第二人民医院病理科,福州350003 [2]福建中医药大学中西医结合学院,福州350108 [3]泰州市人民医院内科,江苏泰州225300

出　　处：《南昌大学学报（医学版）》2012年第6期6-9,共4页Journal of Nanchang University：Medical Sciences

基　　金：福建省自然科学基金(C0510025)

摘　　要：目的研究石斛合剂对高糖、高脂+STZ造模糖尿病大鼠胰岛细胞凋亡及相关基因Bax、Bcl-2mRNA表达的影响。方法大鼠高脂、高糖饲养1个月后腹腔注射STZ(30mg)制备糖尿病模型,经石斛合剂(7.5、15.0、30.0g.kg-1)治疗2周后检测胰岛细胞凋亡及凋亡相关基因Bax、Bcl-2mRNA的表达。结果石斛合剂可显著地降低糖尿病大鼠空腹血糖(P<0.05或P<0.01);石斛合剂可显著减少糖尿病模型大鼠胰岛细胞凋亡(P<0.05),降低Bax mRNA表达(P<0.05),增加Bcl-2mRNA表达(P<0.05或P<0.01))。结论石斛合剂可降低血糖、减少大鼠胰岛细胞凋亡,可能与其降低糖尿病模型大鼠胰腺组织Bax、增加Bcl-2表达有关。Objective To observe the effects of dendrobium compound on the apoptosis and mRNA expression of bax and bcl-2 in islet cells in diabetes rats.Methods The diabetes was induced by high fat and glucose feeding for 1 month,followed by intraperitoneal injection of streptozotocin（30 mg）.The diabetes rats were treated with dendrobium compound（7.5、15.0、30.0 g·kg-1）for two weeks and the apoptosis and mRNA expression of bax and bcl-2 were detected in islet cells.Results Dendrobium compound significantly decreased fasting blood glucose concentrations,islet cell apoptosis and bax expression,but obviously increased bcl-2 expression（P〈0.05 or P〈0.01）.Conclusion Dendrobium compound can reduce blood glucose concentrations and islet cell apoptosis through decreasing bax expression and increasing bcl-2 expression in pancreatic tissue in diabetes rats.

关 键 词：石斛合剂 细胞凋亡 凋亡相关基因 BAX Bcl-2 糖尿病 动物 实验 大鼠 

分 类 号：R-332[医药卫生]