PTTG基因蛋白的表达与垂体腺瘤侵袭性和增殖能力的关系  

Expression of pituitary tumor transforming gene(PTTG) protein and relations to cell proliferation and invasion in pituitary adenimas

作　　者：沈云龙 崔玉婷 

机构地区：[1]从化市中心医院神经外科,广东广州510000

出　　处：《解剖学研究》2012年第3期202-205,共4页Anatomy Research

基　　金：广州市医药卫生科技项目(201102A213209);广东省医学科学技术研究基金(A2011529)

摘　　要：目的探讨人垂体腺瘤中垂体肿瘤转化基因(PTTG)蛋白的表达与肿瘤侵袭性和增殖程度的关系。方法采用免疫组织化学染色方法检测手术切除石蜡包埋的63例垂体腺瘤(侵袭组45例,非侵袭组18例)组织中PTTG蛋白的表达,染色增殖细胞核抗原(PCNA),同时计数组织内微血管数量(MVD)。结果 PTTG在侵袭性垂体腺瘤中的表达水平显著高于非侵袭性垂体腺瘤。侵袭性垂体腺瘤中PCNA标记指数和微血管密度也显著高于非侵袭性垂体腺瘤。相关分析显示PTTG表达与垂体腺瘤内PCNA标记指数和微血管密度呈正相关(P<0.05)。结论 PTTG在垂体腺瘤形成过程中起重要作用,并与垂体腺瘤的侵袭性和增殖程度密切相关。Objective To investigate the role of pituitary tumor transforming gene （PTFG） protein on tumor invasiveness and cell preoliferation in human pituitary adenomas. Methods Immunohistochemical techniques were used to detect the expression of pi- tuitary tumor transforming gene （PTTG） protein in 63 cases of surgical removed and paraffin-embedded pituitary adenomas （45 cases of invasive, 18 cases of non-invasive tumors）. A polyclonal antibody against human factor Ⅷ related antigen were used to identify mi- crovascular density （MVD） and a monoclonal antibody against proliferating cell nuclear antigen （PCNA） was used as a marker for proliferating. Results PTTG protein were significantly increased in invasive pituitary adenomas than in non- invasive group. PCNA labelling index and MVD in invasive group were also significantly higher than non- invasive group. And the expression of PTTG pro- tein was positively correlated to PCNA labelling index and MVD. Conclusions PTTG may play important roles in early pituitary tu- moregenesis and correlate with its invasiveness and cell proliferation.

关 键 词：垂体腺瘤 侵袭性 增殖 垂体肿瘤转化基因 

分 类 号：R736.4[医药卫生—肿瘤]