氨基甾体H42649对K562白血病细胞的抑制增殖和诱导分化作用  

Proliferation and differentiation inducing effects of aminosteroid H42649 on K562 leukemia cells

作　　者：张君[1] 周阳[1] 何群[2] 

机构地区：[1]怀化医学高等专科学校,湖南怀化418000 [2]中南大学基础医学院生理学系,湖南长沙410078

出　　处：《解剖学研究》2012年第3期215-219,共5页Anatomy Research

基　　金：国家自然科学基金(30470638)

摘　　要：目的观察氨基甾体H42649对人慢性粒细胞白血病K562细胞系的抑制增殖和诱导分化作用。方法采用液体培养实验,MTT实验,集落培养实验观察10-8～10-4mol/L浓度的H42649对K562细胞增殖能力的影响;采用Wright-Giemsa染色,联苯胺染色和流式细胞术评价10-6mol/L浓度的H42649对K562细胞的诱导分化作用。结果不同浓度的H42649连续作用K562细胞1～5 d后,细胞计数和集落计数明显减少;第5 d处理组的MTT值显著低于对照组,并呈剂量依赖关系;10-6mol/L浓度的H42649对K562细胞作用5d后,联苯胺染色A值升高(P<0.01),形态学观察其趋向成熟分化,流式细胞术检测药物处理4 d后K562细胞膜上CD71表面标记表达阳性率为84%。结论氨基甾体H42649能显著抑制K562细胞的增殖并诱导其向红系分化,提示该药可作为一种新型慢粒白血病细胞的诱导分化剂。Objective To explore the proliferation and differentiation- inducing effects of aminosteroid H42649 on human chronic myelogenous leukemia K562 cell line. Methods The effects of H42649 on K562 cells proliferation were studied by cell count, colony count and MTT assay. Differentiation was measured by morphology, benzidine stain and flow cytometry. Results The growth of K562 cells were inhibited after treatment with 10^-8-10^-4 mol/L H42649 for 5 days. The inhibition rates for K562 cells in MTT assay was （55.26＋2.54）% after treatment by 10^-6 mol/L H42649 for 5 days. H42649 also induced K562 cells toward erythroid differentiation. It was verified by bezindine stain that the A value at the dose of 10^-6 mol/L H42649 for 5 days was increased （0.2701±0.1960） % compare with the control. The morphology showed the differentiation tendency after treatment of K562 cells. Data from flow cytometry showed that after treatment with 104 mol/L H42649 for 4 days, the percentage of positive CDT1 expression in differentiated K562 cells was 84%. Conclusion Aminosteroid H42649 inhibited the proliferation of K562 cells in a dose-dependent manner. Aminosteroid H42649 induced K562 toward erythroid differentiation.

关 键 词：氨基甾体 K562细胞系 增殖 分化 

分 类 号：R733.7[医药卫生—肿瘤]