肺炎军团杆菌诱导NCI-H292细胞β-defensin-2表达的分子机制  

Molecular mechanism of β-defensin-2 expression in Legionella pneumoniae induced NCI-H292 cells

作　　者：佟爱华[1] 米运强[1] 李康[1] 李艳艳[1] 

机构地区：[1]解放军第309医院检验科,北京100091

出　　处：《武警医学》2012年第7期590-592,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨人β防御素2(human beta defensin 2,hBD-2)基因作用的分子机制,为临床抗感染治疗提供依据。方法肺炎军团杆菌感染NCI-H292细胞,采用菌落形成实验,Western blot实验和ELISA实验检测丝裂原活化蛋白激酶(mi-togen-activatived protein kinase,MAPK)途径内基因的变化。结果 hBD-2对肺炎军团杆菌有抑制作用,肺炎军团杆菌诱导分泌hBD-2主要是通过JNK和p38途径起作用。结论肺炎军团杆菌主要通过c-Jun氨基末端激酶(the c-Jun N-termi-nal kinase,JNK)和p38途径诱导NCI-H292细胞分泌hBD-2。Objective To investigate the role of hBD-2 gene for the clinical anti-infective therapy.Methods Legionella pneumoniae infection in NCI-H292 cells,using colony formation assay,Western blotting,and ELISA to investgate the related gene changes in the MAPK pathway.Results hBD-2 inhibited Legionella pneumoniae,and Legionella pneumoniae induced secretion of hBD-2 mainly through the JNK and p38 pathway.Conclusions Legionella pneumoniae induces hBD-2 secreted from NCI-H292 cells,which is mainly due to JNK and p38 pathway.

关 键 词：军团杆菌 人Β防御素2 丝裂原活化蛋白激酶 

分 类 号：R738.1[医药卫生—肿瘤]