电离辐射对角膜新生血管形成的抑制作用  

Effect of 90Sr-90y ophthalmic applicator on corneal neovascularization after alkali burn ZHOU Hong-yan,MA Qing-fie,HAO Ji-long, GU Shu-yan, GAO Shi, WEN Qiang, ZHAO Yan-ying, ZHANG Wen-song. Department of Ophthalmology, China-Japan Union Hospital, Jilin University, Changchun 130033, China

作　　者：周鸿雁[1] 马庆杰[2] 郝继龙[1] 谷树严[1] 高识[2] 温强[2] 赵艳颖[3] 张文松[4] 

机构地区：[1]吉林大学中日联谊医院眼科,长春130033 [2]吉林大学中日联谊医院核医学科,长春130033 [3]吉林大学中日联谊医院放射线科,长春130033 [4]吉林大学第二医院眼科医院青光眼科,长春130041

出　　处：《中华实验眼科杂志》2012年第8期686-691,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：吉林省科技厅国际合作项目（20120726）;吉林大学基本科研业务费资助项目（450060481914）

摘　　要：背景角膜新生血管（CNV）可发生在多种眼表疾病中，常可加重病情，但有效的I临床治疗方法仍在探索中。目的探讨电离辐射对角膜碱烧伤后新生血管的抑制作用。方法76只清洁级Wistar纯系大鼠中70只用角膜碱烧伤的方法建立大鼠右眼CNV模型，然后按照随机数字表法将造模动物分为B射线10Gy一次性照射组2只、β射线7Gy分次照射组17只、β射线10Gy分次照射组17只、质量分数1％环孢素A（CsA）点眼组17只和角膜碱烧伤模型组17只，6只正常兔6只眼（均取右眼）作为正常对照组。用90Sr-90Y眼科敷贴器于右眼角膜碱烧伤后第1天开始沿角膜缘进行B射线照射，1％CsA点眼组用药时间与照射时间相同。实验后每日行裂隙灯检查并计算CNV长度和面积。于角膜碱烧伤后3、5、7d制备角膜组织石蜡切片和匀浆，采用免疫组织化学法检测各组大鼠角膜组织中bcl-2、bax、血管内皮生长因子（VEGF）的表达，分别采用Westernblot和逆转录聚合酶链反应（RT—PCR）检测各组大鼠角膜组织中VEGF蛋白及mRNA表达的变化。结果角膜碱烧伤后7d，裂隙灯下可见β射线10Gy一次性照射组、B射线10Gy分次照射组均出现角膜溃疡，角膜碱烧伤模型组可见大量CNV生成，而β射线7Gy分次照射组和1％CsA点眼组CNV明显较少。角膜碱烧伤后7d，β射线7cy分次照射组、1％CsA点眼组CNV长度和面积均明显小于角膜碱烧伤模型组，差异均有统计学意义（长度：q=14．40、24．20，P〈0．01；面积：q=17．80、14．00，P〈0．01）。免疫组织化学检测表明，与角膜碱烧伤模型组比较，β射线7Gy分次照射组、1％CsA点眼组大鼠角膜中bcl-2和VEGF蛋白表达均减弱，而bax蛋白表达均增强。RT—PCR检测表明，β射线7Gy分次照射组、β射线10Gy分次照射组、1％CsA点跟组VEGFmRNA表达强度明显低于角膜碱烧伤模型组，Westernblot检测发现VEGF蛋白的表�Background Corneal neovascularization （CNV） is a complication of many ocular surface diseases. It often worsen the pathological course. Effective therapy for CNV is still researching. Objective This study was to investigate the inhibitory effect of irradiation on CNV. Methods CNV models were established in 70 right eyes of 70 clean Wistar rats by corneal alkali burning. The models were randomized into β ray 10 Gy once irradiation group（2 eyes）, β ray 7 Gy muhiple irradiation group（ 17 eyes）, β ray 10 Gy multiple irradiation group（ 17 eyes） , 1% eyclosporin A （CsA） eye drops group （ 17 eyes） and model group （ 17 eyes） , and 6 matched normal rats were used as normal controls. All treatments started from the first day of the corneal alkali burning. CNV length and area were measured under the slit lamp every day. Corneal samples and homogenate were prepared 3,5,7 days after corneal alkali burning. The expressions of bcl-2 ,bax, vascular endothelial growth factor（VEGF） in rat corneas were detected by immunochemistry, VEGF proteins and VEGF mRNA were detected by Western blot and reverse transcription polymerase chain reaction （RT-PCR） , respectively. Results Corneal ulceration was found in the β ray 10 Gy once irradiation group and β ray 10 Gy muhiple irradiation group. CNV length and area were much less in the β ray 7 Gy multiple irradiation group and 1% CsA eye drops group compared with the model group on the seventh day after experiment （ length ： q = 14.40,24.20, P〈 0.01 ; area ： q = 17.80,14.00, P 〈 0.01 ）. Immunochemistry revealed that compared with the model group,expressions of bcl-2 and VEGF proteins were weaker,but the expression of bax protein was stronger in the β ray 7 Gy muhiple irradiation group and 1% CsA eye drops group. RT-PCR showed that the expression of VEGF mRNA in cornea was lower in the β ray 10 Gy multiple irradiation group, β ray 7 Gy multiple irradiation group and 1% CsA eye drops group in comparison with that in model group,and the res

关 键 词：电离辐射 角膜碱烧伤 角膜新生血管 90Sr-90Y眼科敷贴器 

分 类 号：R779.1[医药卫生—眼科]