成人骨髓多能成体祖细胞生物学性状及向成软骨样细胞的诱导分化(英文)  

Biological characteristics and chondrogenic differentiation of bone marrow-derived multipotent adult progenitor cells

作　　者：温昱[1] 范时祥[2] 易先宏[2] 李彬[3] 潘骏[2] 

机构地区：[1]中国医科大学组织胚胎学教研室,辽宁省沈阳市200433 [2]温州医学院附属第二医院,浙江省温州市325027 [3]中国医科大学附属盛京医院骨科,辽宁省沈阳市200433

出　　处：《中国组织工程研究》2012年第27期4968-4973,共6页Chinese Journal of Tissue Engineering Research

基　　金：the Natural Science Foundation of Zhejiang Province,No. Y2100899;Project of Science Technology Department of Zhejiang Province,No. 2009C33131;Project of Whenzhou Science and Technology Bureau,No. H20080057~~

摘　　要：背景:骨髓多能成体祖细胞具有比骨髓间充质干细胞更强的分化潜能和扩增能力并具有胚胎干细胞样的多向分化潜能,但分化为成软骨样细胞的研究目前少见报道。目的:观察成人骨髓成体多能祖细胞的体外培养条件、生物学特性及其向成软骨样细胞分化的可能性。方法:观察体外培养成人骨髓成体多能祖细胞的形态、生长情况,并鉴定;以分化培养基诱导成人骨髓成体多能祖细胞向无软骨细胞分化。结果与结论: 光学显微镜下观察培养的成人骨髓多能成体祖细胞体积偏大,长多角型,数个细长伪足,细胞核大,细胞质丰富,细胞增殖能力旺盛;可见转录因子 4、阶段特异性胚胎表面抗原 1表达,未见 T 细胞表面黏附分子 CD44,CD45,CD133 和主要组织相容性抗原系统Ⅱ的表达;诱导分化后,分化细胞经甲苯胺蓝染色后呈异染性,可表达Ⅱ型胶原。证实,实验成功体外培养了成人骨髓多能成体祖细胞,骨髓多能成体祖细胞在一定诱导条件下具有向成软骨样细胞分化的潜能。BACKGROUND： Bone marrow-derived multipotent adult progenitor cells have stronger differentiation and expansion potential than bone marrow mesenchymal stem cells and exhibit the multiple differentiation potential of chondroblast-like cells. But the reports on the differentiation into chondroblast-like cells are rare. OBJECTIVE： To study the culture and characteristics of bone marrow-derived multipotent adult progenitor cells and the potential to differentiate into chondroblast-like cells. METHODS： The bone marrow-derived multipotent adult progenitor cells were cultured in vitro, and then the morphology and the growth conditions were observed and identified. The differentiation medium was used to introduce the bone marrow-derived multipotent adult progenitor ceils to differentiate into chondroblast-like cells. RESULTS AND CONCLUSION： Observation under the light microscope showed that bone marrow-derived multipotent adult progenitor cells were large and grew in long polygonal with several slender pseudopodia, the cells were with large nucleus and affluent cytoplasma and had strong proliferation ability; the expression of transcription factor 4 and stage-specific embryonic antigen 1 could be seen, the expressions of T-cell surface adhesion molecules of CD44, CD45 and CD133 and major organizations compatibility antigen systems Ⅱ were not shown. After induction and differentiation, toluidine blue stained differentiated cells showed metachromasia and could express collagen type Ⅱ. The bone marrow-derived multipotent adult progenitor cells were successfully cultured in vitro, and the bone marrow-derived multipotent adult progenitor cells could differentiate into the chondroblast-like cells in a certain induction conditions.

关 键 词：多能成体祖细胞 骨髓 细胞培养 细胞分化 成软骨样细胞 骨髓来源干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]