成骨诱导脂肪基质细胞在骨组织工程体内成骨中的作用  

Osteogenic induction of adipose derived stromal cells in bone tissue engineering in vivo

作　　者：李晓宇[1] 姚金凤[2] 刘政华[1] 蔡剑波[1] 

机构地区：[1]深圳市宝安区人民医院口腔颌面外科,广东省深圳市518101 [2]深圳市第二人民医院口腔科,广东省深圳市518035

出　　处：《中国组织工程研究》2012年第27期4985-4990,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:以往研究认为,经过成骨诱导后的脂肪基质细胞通过转化为成骨细胞分泌骨基质进而修复骨缺损,然而并没有明确结论证实。目的:将经过体外成骨诱导的脂肪基质细胞复合支架材料分别植入骨缺损区和非骨区,根据是否成骨,验证经过成骨诱导后的脂肪基质细胞是否转化为成骨细胞。方法:取12月龄犬背部皮下脂肪,经胶原酶消化法获得单个核细胞,将培养的第3代细胞与双相磷酸钙陶瓷形成复合物。在犬下颌骨两侧制备长20mm、高10mm的箱状缺损,拔除术区牙齿,将细胞支架复合物植入一侧术区,空白侧留作对照;另外在犬背部皮下肌肉区植入细胞支架复合物及骨诱导性磷酸钙陶瓷材料,术后6周及12周经组织学检测骨缺损修复情况。结果与结论:脂肪基质细胞复合双相磷酸钙陶瓷在骨缺损区成骨,在肌肉区未形成新骨;骨诱导性磷酸钙陶瓷在肌肉区形成新骨。提示成骨诱导并不能将脂肪基质细胞转化为成骨细胞,其确切机制有待进一步研究。BACKGROUND： Osteogenic induction has been regarded as an indispensable step for adipose-derived stromal cells （ADSCs） to transform into osteoblasts, thus to secret bone matrix to repair bone defects. However, this conclusion has not been confirmed. OBJECTIVE： Tissue-engineered constructs were prepared from osteogenically induced ADSCs and scaffolds. The constructs were implanted into bone defects and non-bone areas. Whether osteogenically induced ADSCs were transformed into osteoblasts was determined according to bone formation. METHODS： ADSCs were prepared by collagenase 1 digestion of subcutaneous fat from the back of dogs aged 12 months. Passage 3 ADSCs were harvested and seeded on the biphasic calcium phosphate ceramics to form a cell-scaffold construct. Box-like bone defects, 20 mm in length and 10 mm in height, were created in the mandibular bilaterally in each dog along the alveolar bone where teeth had been extracted. Then the cell-scaffold constructs were implanted in dog mandibular bone defects. An empty defect group was used as control. The tissue-engineered constructs and osteoinductive calcium phosphate ceramics were implanted in muscle. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry. RESULTS AND CONCLUSION： ADSCs-scaffold construct promoted bone formation in bone defect area, but not in muscle area, however, osteoinductive calcium phosphate ceramics promoted bone formation in muscle area. ADSCs were not transformed into osteoblasts after osteogenic induction. The precise mechanism needs further investigation

关 键 词：脂肪基质细胞 双相磷酸钙陶瓷 成骨诱导 骨缺损 组织工程 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]