表皮干细胞与毛囊干细胞生物学特性的比较  

Biological characteristics of epidermal stem cells and follicle stem cells

作　　者：杨青[1] 李秀兰[2] 张杨[2] 郭悦[2] 孙晓雷[2] 

机构地区：[1]天津医科大学,天津市300070 [2]天津医院骨科研究所,天津市300211

出　　处：《中国组织工程研究》2012年第27期5034-5038,共5页Chinese Journal of Tissue Engineering Research

基　　金：天津市科技支撑计划重点项目(08ZCGYSF01300);天津市自然科学基金项目(11JCYBJC10200)~~

摘　　要：背景:获取更合适的组织工程皮肤种子细胞可使皮肤功能得到更好的修复。目的:分离、培养表皮干细胞和毛囊干细胞,并比较两种细胞的生物学特性。方法:体外分离培养2月龄新西兰兔表皮干细胞和毛囊干细胞,取生长良好的第2,3,6代细胞观察其生物学特性。结果与结论:毛囊干细胞较表皮干细胞贴壁快,具有更高的增殖活性。免疫组化及荧光定量PCR检测显示毛囊干细胞β1整合素、角蛋白19蛋白及mRNA表达均明显高于表皮干细胞。提示作为皮肤组织工程的种子细胞,毛囊干细胞较表皮干细胞更具优势。BACKGROUND： More appropriate skin tissue engineering seed cells can better repair skin function OBEJECTIVE： To isolate and culture epidermal stem cells and follicle stem cells, and compare their biologica characteristics. METHODS： Epidermal stem cells and follicle stem cells of 2-month-old New Zealand rabbits were isolated and cultured in vitro. The biological characteristics of passage 2, 3, 6 cells which grew well were observed. RESULTS AND CONCLUSIONS： Follicle stem cells attached faster and had higher proliferative capacity than epidermal stem cells. Immunohistochemical staining and fluorescent quantitative PCR showed that the follicle stem cells showed higher protein and mRNA expression of β1 integrin and K19 than epidermal stem cells. These findings suggest that follicle stem cells, as seed cells of skin tissue engineering, are superior to epidermal stem cells.

关 键 词：表皮干细胞 毛囊干细胞 角蛋白19 Β1整合素 基因 生物学特性 种子细胞 皮肤组织工程 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]