骨髓间充质干细胞分泌细胞因子与腹腔肥大细胞膜的稳定性  

Influence of secreted cytokines of bone marrow mesenchymal stem cells on the stability of rat peritoneal mast cell membrane

作　　者：高明敏[1] 刘晓萍[2] 于业军[1] 贾跃伟 王家辉[1] 李薇[2] 

机构地区：[1]青岛大学医学院附属医院皮肤科,山东省青岛市266003 [2]青岛大学医学院组织胚胎学教研室,山东省青岛市266003 [3]奥克生物开发有限公司,山东省青岛市266003

出　　处：《中国组织工程研究》2012年第27期5052-5056,共5页Chinese Journal of Tissue Engineering Research

基　　金：青岛市科技局资助课题(07-2-1-4-nsh)~~

摘　　要：背景:研究表明骨髓间充质干细胞分泌的细胞因子对特异性和非特异性免疫细胞具有免疫调节作用,所以有必要深入研究其对肥大细胞的作用。目的:探讨骨髓间充质干细胞分泌因子对特异性和非特异性诱发肥大细胞脱颗粒、释放炎性递质的影响。方法:采用全骨髓贴壁法分离和纯化大鼠骨髓间充质干细胞,收集培养第3代的骨髓间充质干细胞上清液获取干细胞分泌因子。分离培养大鼠腹腔肥大细胞,分别以大鼠抗卵蛋白血清特异性被动致敏腹腔肥大细胞和用Compound48/80非特异性致敏肥大细胞。结果与结论:干细胞因子、酮替芬均能抑制特异性和非特异性诱发肥大细胞脱颗粒和释放类胰蛋白酶,与模型对照组相比差异有显著性意义(P<0.05),但干细胞因子抑制特异性和非特异性诱发肥大细胞脱颗粒和释放类胰蛋白酶的效果均不如酮替芬组(P<0.05)。结果表明干细胞分泌的细胞因子具有稳定肥大细胞膜,减轻其脱颗粒、释放炎症递质的作用。BACKGROUND： Mesenchymal stem cells secreted cytokines has immunomodulatory effects on specific and nonspecific immune cells. Whether the cytokines have an effect on mast cells requires further studies. OBJECTIVE： To explore the influence of the cytokines secreted by bone marrow mesenchymal stem cells on the degranulation of specific and nonspecific induced mast cells and secretion of inflammatory mediators. METHODS： Rat bone marrow mesenchymal stem cells were isolated and purified by whole bone marrow adherent method. The stem cell cytokines were obtained from the supernatants of the third generation of bone marrow mesenchymal stem cells. The mast cells were isolated from rat peritoneal mast cells. Mast cells were specifically sensitized by rat antiovalbumin serum and nonspecifically stimulated by Compound48/80. RESULTS AND CONCLUSION： Compared with the control group, cytokines from bone marrow mesenchymal stem cells and ketotifen could significantly inhibit the degranulation of the specific and nonspecific mast cells and release tryptase, and there was significant difference （P 〈 0.05）, however, the effect of cytokines from bone marrow mesenchymal stem cells was weaker than that from the ketotifen （P 〈 0.05）. Cytokines from bone marrow mesenchymal stem cells could stable mast cells membrane, inhibit the degranulation of mast cells and the release inflammatory mediators in mast cells.

关 键 词：肥大细胞 骨髓间充质干细胞 细胞因子 免疫调节 大鼠 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]