慢性缺氧可通过氧化应激和细胞凋亡引起大鼠延髓呼吸中枢的损伤  

Damage Effects of Chronic Hypoxia on Medulla Oblongata Associated with Oxidative Stress and Cell Apoptosis

作　　者：侯雪飞[1] 丁艳[1] 聂政[1] 李慧[1] 唐玉红[1] 周华[1] 陈丽[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041

出　　处：《生物医学工程学杂志》2012年第4期727-731,736,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30770798;30971073)

摘　　要：本实验研究慢性缺氧对大鼠延髓呼吸中枢的损伤作用,并探讨其损伤作用是否与氧化应激和细胞凋亡有关。健康雄性SD大鼠,随机分为两组:空气对照组和慢性缺氧组。采用尼氏染色法,观察慢性缺氧对延髓呼吸中枢神经元的损伤;采用生物化学方法,检测延髓丙二醛(MDA)的含量和超氧化物歧化酶(SOD)的活力,观察慢性缺氧对延髓氧化应激水平的影响;采用RT-PCR方法,检测延髓Bax mRNA和Bcl-2mRNA的表达,观察慢性缺氧对延髓细胞凋亡水平的影响。结果显示:与空气对照组相比,在慢性缺氧组大鼠延髓,pre-BtC、Amb、NTS、FN、12N等呼吸相关核团尼氏染色光密度值均降低(P<0.05);MDA含量增加(P<0.05),SOD活力无显著变化(P>0.05);Bcl-2mRNA水平降低(P<0.05),Bax mRNA水平无显著差异(P>0.05)。研究表明,慢性缺氧对呼吸中枢有严重损伤,这种损伤可能与其增强氧化应激和促进细胞凋亡等作用有关。The aim of this study is to study the damage effects of chronic hypoxia on medulla oblongata and to explore whether the damage is associated with oxidative stress and cell apoptosis. Adult male SD rats were randomly divided into two groups： control group and chronic hypoxia group. Medulla oblongata was obtained for the following meth- ods of analyses. Nissl＇s staining was used to examine the Nissl bodies of neurons in medullary respiratory related nu- clei, biochemistry methods were utilized to examine oxidant stress damage induced by chronic hypoxia on medulla ob- longata through measuring malondialdehyde （MDA） content and superoxide dismutase （SOD） activity, and RT-PCR technique was used to study the influence of apoptosis induced by chronic hypoxia on medulla oblongata through ana- lyzing the levels of Bax mRNA and Bcl-2 mRNA. The results showed the optical densities of Nissl＇s staining in pre- B6tC, NA, NTS, FN, and 12N were significantly decreased in chronic hypoxia group in comparison with that in control group （P^0.05）. In chronic hypoxia group, MDA level was significantly higher than that in the control group （P〈0.05） , whereas SOD level had no significant difference between the two groups（P^0. 05）. Bax mRNA expression had no obvious change and Bcl-2 mRNA expression significantly decreased in chronic hypoxia group in comparison with that in control group （P〈0. 05）. The results suggest that chronic hypoxia could bring about serious damage to medullary respiratory centers through aggravating oxidative stress and increasing cell apoptosis.

关 键 词：慢性缺氧 呼吸中枢 延髓 氧化应激 细胞凋亡 

分 类 号：R363[医药卫生—病理学]