17-AAG对转染NIS基因的未分化甲状腺癌摄碘动力学的影响  

The effect of 17-AAG on iodine uptake kinetics of NIS-transfected anaplastic thyroid cancer cells

作　　者：王任飞[1] 谭建[1] 李玮[1] 孟召伟[1] 郑薇[1] 

机构地区：[1]天津医科大学总医院核医学科,300052

出　　处：《中华核医学与分子影像杂志》2012年第4期281-285,共5页Chinese Journal of Nuclear Medicine and Molecular Imaging

摘　　要：目的探讨17-丙烯胺基-17-去甲氧基格尔德霉素（17-AAG）对已转染NIS基因的未分化甲状腺癌（ATC）细胞摄碘动力学的影响。方法采用脂质体转染法，将携带NIS基因的重组质粒pcDNA3．1-NIS转染入ATC细胞株FRO细胞中，G418抗性筛选获得稳定表达细胞系NIS—FRO。在NIS—FRO细胞的培养基中引入^125I，进行^125I内流及外流的系列实验，绘制时间-放射性曲线。进一步分析经1Ixmol／L的17-AAG作用24h后NIS—FRO细胞^125I内流及外流的变化，并与未经转染的细胞进行对比，2组间各个时间点的放射性计数采用两样本t检验进行统计学分析。结果NIS．FRO细胞的摄^125I能力明显提高，约为FRO细胞的10．68倍（t=45．329，P〈0．001），但去除孵育环境中的^125I后30min，细胞内的125I滞留率仅为初始的10．5％。1ixmol／L的17-AAG作用于NIS—FRO细胞后24h，细胞摄125I能力进一步提高，在含^125I的培养基中孵育20—60min，其放射性计数为31771．8～54815．5min-1，摄125I能力较对照组（24020．3～41293．8）提高了24．8％～35．5％（t值依次为3．096、4．275、3．055、4．292和5．496，P均〈0．05）；撤掉孵育环境中的125I后5～30min，细胞内的125I滞留率为32．7％～85．2％，较对照组（10．5％～56．8％）明显增加（t值依次为22．801、13．096、19．631、38．205、43．519和29．322，P均〈0．01），125I的外流减少，30min后17-AAG作用组的细胞内125I滞留率为32．7％，为对照组的3．1倍。结论把NIS转染入ATC细胞后获得的稳定表达细胞株在经一定浓度的17-AAG作用后，可进一步提高肿瘤细胞的摄碘率，并可明显延迟碘的外流，提高细胞内碘的滞留率。Objective To investigate the effect of 17-allylamino-17-demethoxy geldanamycin （ 17- AAG） on iodine uptake kinetics of NIS-transfected anaplastic thyroid cancer （ATC） cells. Methods Lipofection was used to transfeet the recombinant plasmid, namely peDNA3.1-NIS, into FRO cells （ATC cell line）. A stable cell line NIS-FRO was obtained by G418 resistance selection. 125I was added into the medium, and influx and efflux experiments were performed. Different time-radioactivity curves were drawn, and further a- nalysis was performed between the non-transfected cells （the control group） and NIS-FRO cells treated with 1 μmol/L 17-AAG for 24 h. Student＇s t-test was used to analyze the data. Results The iodine uptake a- bility of the NIS-FRO cells was significantly higher than that of the FRO cells （ about 10.68 times, t = 45. 329, P 〈 0. 001 ）. However, 125I out-flowed rapidly when removed from the medium, and the retention rate of 125I in the NIS-FRO cells was only 10.5% of the initial amount after 30 min. After treatment with 1 μmol/L 17-AAG for 24 h, the 125I uptake ability of NIS-FRO cells further increased. During the 20 - 60 min incuba- tion with 125I, the iodine uptake ability of 17-AAG treated MS-FRO cells increased significantly with radio- active counts of 31771.8 - 54815.5 per minute, which was much higher than that of the control group （ 24020.3 - 41293.8 per minute ; t = 3. 096, 4.275, 3. 055, 4.292 and 5. 496, respectively, all P 〈 O. 05）. The iodine uptake ability increased about 24.8%-35.5%. Furthermore, 5- 30 min after removing the medi- um, the retention rates of 125I in the 17-AAG treated NIS-FRO cells were significantly increased compared with those of the control group （32.7%-85.2% vs 10.5%-56.8%; t =22.801, 13. 096, 19. 631, 38. 205, 43. 519, 29. 322, respectively, all P 〈 0.01 ） , and 125I efflux was reduced. After 30 rain, 125I re-tention rate of the treatment group was 32.7%, which was 3.1 times higher than that of the control group. Conclusion The iodine uptake

关 键 词：甲状腺肿瘤 转染 钠/碘转运体 AAG 动力学 

分 类 号：R736.1[医药卫生—肿瘤]