奥曲肽对人胆管癌QBC939裸鼠种植瘤的抑制作用  

Inhibitory effect of octreotide on growth of implanted tumor of human cholangiocarcinoma QBC939 cells in nude mice

作　　者：陈有挺[1] 周智[2] 石铮[1] 林永堃[1] 

机构地区：[1]福建医科大学附属第一医院肝胆胰腔镜微创外科,福建福州350005 [2]福建医科大学附属漳州市医院普通外科,福建漳州363000

出　　处：《中国普通外科杂志》2012年第8期952-956,共5页China Journal of General Surgery

基　　金：福建省教育厅科技项目(JA09119)

摘　　要：目的:探讨奥曲肽(OCT)对人胆管癌QBC939裸鼠种植瘤的作用及其可能的机制。方法:建立人胆管癌QBC939裸鼠种植瘤模型12只,随机均分为实验组和对照组,分别皮下注射OCT[100μg/(kg.d)]和等体积生理盐水,1次/d,连续4周,最后1次给药24 h后处死裸鼠,完整剥除瘤块,测量瘤体体积和重量,计算实验组抑瘤率;采用免疫组织化学方法分别检测缺氧诱导因子1α(HIF-1α)在两组瘤组织中的表达情况及计数微血管密度(MVD);应用TUNEL法检测两组瘤组织凋亡情况。结果:实验组的种植瘤体积和重量明显小于对照组[(1934.85±272.88)mm3 vs.(2834.63±521.86)mm3;(1.77±0.23)g vs.(2.44±0.65)g](均P<0.05),实验组抑瘤率为29.20%;实验组瘤组织中HIF-1α的表达量和MVD况均明显少于对照组[(0.2605±0.0406)vs.(0.3284±0.1008);(13.61±1.87)vs.(17.44±2.24)](均P<0.05);实验组瘤组织的凋亡指数(AI)明显大于对照组(P<0.05)。结论:OCT对人胆管癌裸鼠种植瘤生长具有抑制作用,其机制可能与诱导肿瘤细胞凋亡和抑制肿瘤血管生成有关。Objective： To investigate the effect of octreotide on the subcutaneously implanted tumor mass derived from human cholangiocarcinorma Q.BC939 cells in nude mice and its possible mechanism. Methods. Twelve nude mice bearing tumor mass composed of human cholangiocarcinorma cancer QBC939 cells were equally randomized into the experimental group and control group. Mice were given respectively octreotide of 100 μg/（kg.d） or normal saline of the same volume by subcutaneous injection with a once-daily regimen for 4 weeks. Mice were sacrificed 24 h after the last dose injection and the tumor masses were resected en bloc, The volumes and weights of the tumors were measured, and tumor inhibition rate of the experiment group was calculated, The expression ofhypoxia-inducible factor 1α （HIF-1 α） and microvessel density （MVD） in tumor tissues of both groups were detected by immunohistochemicaI method. The apoptosis in tumor tissues of both groups was detected by TUNEL assay. Results. The size and weight of the implanted tumor in experiment group were significantly reduced compared with control group [（1934.85±272.88） mm3 vs. （2834.63±521.86） mm3; （1.77±0.23） g vs. （2.44±0.65） g] （both P〈0.05）, and the tumor inhibition rate of experiment group was 29.20%. The expression level of HF- 1α and MVD in the tumor tissues of experiment group were significantly less than those of control group [（0.2605±0.0406） vs. （0.3284±0.1008）; （13.61±1.87） vs. （17.44±2.24）] （both P〈0.05）. The apoptotic index （M） of tumor issues in experiment group was significantly higher than that in control group （P〈0.05）. Conclusion： Octreotide has inhibitory effect on the growth of implanted tumor of human cholangiocarcinoma in nude mice. The mechanism may be relevant to induction of apoptosis and inhibition of antiangiogenesis in tumor cells.

关 键 词：胆管肿瘤 奥曲肽 细胞增殖 细胞凋亡 小鼠 裸 

分 类 号：R735.8[医药卫生—肿瘤]