Illumina Sequencing Technology as a Method of Identifying T-DNA Insertion Loci in Activation- Tagged Arabidopsis thaliana Plants  

作　　者：Joanna K. Polko Mohamed-Ramzi Temanni Martijn van Zanten Wilbert van Workum Sven Iburg Ronald Pierik Laurentius A.C.J. Voesenek Anton J.M. Peeters 

机构地区：[1]Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands [2]ServiceXS BV, Plesmanlaan ld, 2333 BZ Leiden, The Netherlands [3]Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute (MPRI), 3128 Biosciences Research Bldg, University of Maryland, College Park, MD 20742, USA [4]Molecular Plant Physiology, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

出　　处：《Molecular Plant》2012年第4期948-950,共3页分子植物（英文版）

摘　　要：Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are frequently employed. These populations are gener- ated using vectors containing multiple copies of the constitutive 35S promoters derived from cau and often result in isolation i flower mosaic virus （35S CaMV） of dominant gain-of-function alleles （Weigel et al., 2000; Nakazawa et al., 2003）. This allows the study of members of large gene families that are often func- tionally redundant and, therefore, hard to identify in loss-of- function screens. Moreover, due to the dominant nature,Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are frequently employed. These populations are gener- ated using vectors containing multiple copies of the constitutive 35S promoters derived from cau and often result in isolation i flower mosaic virus （35S CaMV） of dominant gain-of-function alleles （Weigel et al., 2000; Nakazawa et al., 2003）. This allows the study of members of large gene families that are often func- tionally redundant and, therefore, hard to identify in loss-of- function screens. Moreover, due to the dominant nature,

关 键 词：T-DNA 激活标签 拟南芥 测序技术 插入位点 35S启动子 识别 中国农业大学 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]