次声作用对大鼠血浆SOD、MDA、NO水平的影响  

Effects of Infrasound on the Levels of SOD,MDA and NO in Rat Plasma

作　　者：张歆薇[1] 刘海强[1] 徐胜龙[1] 周艳[1] 郭国祯[1] 王晋[1] 丁桂荣[1] 

机构地区：[1]第四军医大学军事预防医学院放射医学教研室,陕西西安710032

出　　处：《现代生物医学进展》2012年第18期3416-3419,共4页Progress in Modern Biomedicine

基　　金：国家973项目(2011CB503704;2011CB503705);国家自然科学基金项目(81072272;60871068)

摘　　要：目的:通过观察16 Hz/130 dB次声作用大鼠不同时间其血浆超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平的变化,揭示次声作用对大鼠氧化应激损伤的特点。方法:80只SD大鼠随机分为次声作用1、7、14、21和28 d组及对应的假暴露组,每组8只大鼠。次声暴露组每日定时在次声舱内接受16 Hz/130 dB次声作用2h,连续1、7、14、21和28 d,假暴露组除不施加次声作用外,其在次声舱中放置的时间、次数、采样时间点均和次声暴露组相同。次声暴露后即刻测定大鼠血浆SOD、MDA、NO水平,测得的结果与假暴露组进行比较。结果:与假暴露组相比,大鼠血浆SOD活力在次声作用后显著降低(P<0.05),且随次声作用时间延长,SOD活力逐渐降低;大鼠血浆MDA含量在次声作用后显著升高(P<0.05),且随次声作用时间延长,MDA含量逐渐升高;大鼠血浆NO含量在次声作用7、14 d时显著降低(P<0.05),1、21和28 d时无统计学差异(P>0.05)。结论:次声作用可引起大鼠氧化应激损伤,损伤的程度与次声暴露时间有关。Objective： To observe the effects ofinfrasound on the levels of SOD, MDA and NO in rat plasma. Methods： 80 SD rats were randomly divided into five sham groups and five infrasound exposed groups （n=8）. The rats in exposed groups were exposed under infrasound of frequency at 16 Hz, sound pressure level at 130 dB for 1, 7, 14, 21 and 28 d, 2 h per day, the rats in sham groups were treat- ed the same except infrasound exposure. The levels of SOD, MDA and NO in plasma were measured immediately after final exposure. Results： Comparing to sham groups, the activety of SOD in rat plasma decreased significantly after infrasound exposure for 1, 7, 14, 21 and 28 d （P〈0.135）, the content of MDA in rat plasma increased significantly after infrasound exposure for 1, 7, 14,21 and 28 d （P〈0.05）, the content of NO in plasma decreased significantly at 7 d （P〈0.05）and 14 d （P〈0.05）, at 0, 21 and 28 d normally （P〉0.05）. Conclusion： Infrasound could induce oxidative stress in a time-dependent manner in rats.

关 键 词：次声 大鼠 超氧化物歧化酶 丙二醛 一氧化氮 

分 类 号：R-363[医药卫生]