幽门螺杆菌毒力基因型与胃癌的相关性研究  

The Correlation between Virulence Genotypes of Helicobacter Pylori and Gastric Cancer

作　　者：黄敬敬[1] 魏良洲[2] 战淑慧[1] 董开芯[1] 王传芳[1] 苏艳华[1] 董全江[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院消化内科及中心实验室,山东青岛266071 [2]青岛大学医学院附属医院,山东青岛266071

出　　处：《现代生物医学进展》2012年第18期3525-3528,共4页Progress in Modern Biomedicine

摘　　要：目的:研究中国东部地区幽门螺杆菌(H.pylori)cagA、vacA和iceA1毒力基因型的分布状况及其与胃癌的相关性。方法:从52例病人胃黏膜活检组织(31例慢性浅表性胃炎,21例胃癌)中分离培养H.pylori,用PCR方法扩增分离菌株的cagA、iceA1、vacAs,i,m区毒力基因片段,统计并分析上述毒力因素与胃癌发生的相关性。结果:在52例菌株中,cagA、vacAs1/i1/m1、vacAs1/i1/m2和iceA1的阳性率分别是92.3%(48/52),48.1%(25/52),48.1%(25/52),90.4%(47/52);所有的胃癌分离株中均为cagA(+)vacAs1/i1(+)型,vacAm1、vacAm2和iceA1在胃癌组中的阳性率分别是47.6%(10/21),52.4%(11/21),95.2%(20/21),与胃炎组相比,上述基因型的阳性率差异均无显著统计学意义(P>0.05)。结论:cagA、vacAs1/i1/m1、vacAs1/i1/m2和iceA1是中国东部地区H.pylori的优势基因型;cagA、vacAs1、vacAi1和iceA1毒力基因型的存在与胃癌的发生无相关性。Objective： To investigate the prevalence of virulent genotypes of Helicobacter pylori （H. pylon） and the relationship between virulence factors and gastric cancer. Methods： H. pylori were cultured from gastric biopsies obtained from 31 patients with chronic gastritis and 21 with gastric cancer. The virulence genes, cagA, iceA1, vacA s, i and m regions were amplified by using PCR and the relationship between virulence factors and gastric cancer was analyzed. Results： The prevalence of cagA, vacAsl/il/ml, vacAsl/ il/m2 and iceA1 in 52 isolates were 92.3% （48/52）, 48.1% （25/52）, 48.1% （25/52）, 90.4% （47/52）, respectively. All the gastric cancer isolates were cagA （＋） vacAsl/il （＋）, and the positive rates ofvacAml, vacArn2 and iceA1 in group of gastric cancer were 47.6% （10/21）, 52.4%（11/21）, 95.2%（20/21）, respectively. There were no statistically significant differences in the positive rates of all the genotypes above compared with the positive rates in gastritis isolates （P〉0.05）. Conclusions： The majority of H. pylori isolated from East China possess virulent genotypes are cagA, vacAsl/il/ml, vacAsl/il/m2 and iceA1 positive. It appeared that the virulence factors ofH. pylori had no significant association with gastric cancer.

关 键 词：幽门螺杆菌 CAGA VACA iceA1 毒力 

分 类 号：R735.2[医药卫生—肿瘤]