急性髓细胞性白血病(AML)中IL-3Rα基因的表达及意义  

Expression of IL-3Rα in Acute Myeloid Leukemia(AML) and its Significance

作　　者：宋玉华[1] 彭鹏[1] 邱少敏[1] 王清波[1] 谈国蕾[1] 

机构地区：[1]东南大学医学院附属南京市第二医院肿瘤科,江苏南京210003

出　　处：《现代生物医学进展》2012年第19期3632-3634,3627,共4页Progress in Modern Biomedicine

摘　　要：目的:检测白介素-3受体α亚基(IL-3Rα)在急性髓细胞性白血病(acute myeloid leukemia,AML)中的表达,研究其在AML发病和预后评估中的意义。方法:应用逆转录-聚合酶链反应(RT-PCR)法检测57例AML患者IL-3Rα的表达,其中包括52例初治和复发患者(6例M1、28例M2、10例M3、3例M4、5例M5)及5例缓解患者,另10例正常人作为对照。结果:①10例正常人和5例AML缓解患者未检测到IL-3Rα表达。②52例初治和复发的AML患者中IL-3Rα阳性表达21例,占40.38%,M1～M5各型患者的阳性率差异无显著性(P>0.05)。初治和复发AML患者之间IL-3Rα阳性表达无明显差异(P>0.05)。③AML患者的IL-3Rα阳性表达与外周血白细胞计数、骨髓中原始细胞比例、CD34阳性表达有关(P<0.05)。④AML患者中IL-3Rα阳性表达的患者CR率低于阴性者(P<0.05)。结论:①IL-3Rα在AML发病中有一定作用。②IL-3Rα基因可作为AML预后不良的一个指标。Objective： To investigate the expression and significance of IL-3Rα in 57 paitients with acute myeloid leukemia（AML）.Methods： RT-PCR method was perfomed to examine the expression of IL-3Rα in bone marrow or peripheral blood mononuclear cells from 57 paitients with AML.The paitients included 52 newly diagnosed and relapse paitients（6 M1,28 M2,10 M3,3 M4,5 M5） and 5 CR patients.10 healthy subjects were used as control group.Results： ①5 CR patients didn’t express IL-3Rα mRNA.②The percentage of IL-3Rα mRNA expression in AML was 40.38%.IL-3Rα mRNA was expressed in all AML subtypes,and there was no difference among AML subtypes.In AML paitients,there was no difference between newly diagnosed and relapse patients.③In AML patients,the expression of IL-3Rα mRNA related to white blood count,blast cells ratio and CD34 positive expression（P〈0.05）.④The CR rate of AML patients who expressed IL-3Rα was significantiy lower than that of who didn’t express IL-3Rα（P〈0.05）.Conclusions： ①IL-3Rα gene might play an important role in the pathogenesis of AML.②IL-3Rα gene may be a poor prognostic factor in AML.

关 键 词：白介素-3受体α亚基(IL-3Rα) 急性髓细胞性白血病 

分 类 号：R733.7[医药卫生—肿瘤]