4-NQO饮水法不同给药时间诱发小鼠舌癌模型的比较  

Comparison of mouse tongue epithelial carcinoma model induced by 4-NQO in drinking water in different dosing schedules

作　　者：黎明武[1] 李晶[1] 于大海[1] 卿海云[2] 闭似嫦[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,南宁530021 [2]广西医科大学附属口腔医院病理科,南宁530021

出　　处：《中国临床新医学》2012年第7期581-584,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西教育厅科研项目(编号:桂教科研2003)

摘　　要：目的比较4-硝基喹啉-1-氧化物(4-nitroquinoline 1-oxide,4-NQO)饮水法在不同给药时间诱发小鼠舌癌模型过程中口腔黏膜病变程度的差异。方法 70只balb/c小鼠,随机分为两组:实验组60只,给予200 mg/L 4-NQO饮水喂养8、14、20 W(各20只),停药改用普通自来水喂养到45 W;对照组10只小鼠,单纯饮用自来水。45 W时通过肉眼和组织学观察各组的病变情况并比较小鼠体重的变化情况。结果随着给药时间的延长,实验组小鼠舌背黏膜相继出现白色斑块、溃疡、乳头状增生等改变,小鼠舌背产生轻度异常增生-中度异常增生-重度异常增生-原位癌-鳞癌的典型病理变化。给药8 W组70.6%小鼠舌背黏膜表现为单纯性增生,29.4%为轻中重度异常增生,舌癌发生率为0%;给药14 W组88.2%为轻中重度异常增生,11.8%为原位癌;给药20 W组100%为鳞癌,并且100%转移颈部淋巴结;三组未见远处转移。14、20 W组小鼠体重较给药前明显减轻(P<0.05),8 W组和对照组小鼠体重较给药前无明显变化(P>0.05)。结论在相同剂量下,随着给药时间延长,舌癌前病变以及舌癌发生率越高,但是体重减轻越明显。Objective To compare the degree of oral mucosal lesions of mouse tonglle epithelial carcinoma model induced by four-nitroquinoline 1-oxide （4-NQO） in drinking water in different dosing schedules. Methods Seventy balb/e miee were randomly assigned into two groups ： the experimental group （ n = 60） and tile control group （ n = 10）. The experimental groups were given 200 mg/L 4-NQO in drinking water for 8, 14, 20 weeks （ n = 20） , respectively, and then given ordinary water to 45 weeks. The control group was given ordinary tap water. All the mice were killed in 45 weeks, then their tongues and suhmandihular lymph nodes were removed for histolog/c study. Restilts With the time prolonged, the changes including leukoplakia, ulcer appearances, papillary hyperplasia were gradually occurred on the mucosa of the experimental groups. Their corresponding histopthological results ranged from hyperplasia, mild dysplasia（ MiDP）, moderate dysplasia（ MoDP）, severe dysplasia （SDP）, In situ carcinoma（ISC） to invasive carcinoma（ IC ） along with the increasing administration time. The tongue of mice which were given 4-NQO for 8 weeks showed hyperplasia （70. 6% ） , MiDP/MoDP and SDP （29.4%） ; The tongue of mice which were given 4-NQO for 14 weeks showed MiDP/MoDP and SDP （88.2%）, ISC（ 11.8% ） ; The tongue of mice which were given 4-NQO for 20 weeks showed IC （ 100% ） and 100% submandibular lymph nodes metastasis were found. Compared with before administration, the body weight of mice of 14, 20-week groupss significantly reduced （P 〈 0. 05 ） , while there were no significantly changes of the body weight of mice of 8 weeks and control groups （ P 〉 0. 05 ）. Conclusion 4-NQO could be given in drinking water to induce tongue epithelial carcinoma model at the same dose in different dosing schedules. With the increasing administration time, the higher rate of tongue precancerous lesions and the tongue epithelial carcinoma occured, but the body weight of mice significantly

关 键 词：4-硝基喹啉-1-氧化物 舌癌 小鼠 动物模型 

分 类 号：R739.8[医药卫生—肿瘤]