光敏剂BCPD-17和PSD007对小鼠骨肉瘤的光动力作用  

Photodynamic effects of BCPD-17 and PSD007 on osteosarcoma LM-8 transplanted in mice

作　　者：张治宇[1] 胡硕 蔡郑东[3] 

机构地区：[1]中国医科大学附属第四医院骨科,辽宁沈阳110034 [2]解放军第531医院骨科,吉林通化134002 [3]同济大学附属上海市第十人民医院骨科,上海200072

出　　处：《吉林大学学报（医学版）》2012年第4期644-648,604,共5页Journal of Jilin University：Medicine Edition

基　　金：教育部博士点学科专项基金(新教师类)资助课题(20112104120007)

摘　　要：目的:观察630及670nm激光激发不同剂量的光敏剂BCPD-17和PSD-007对小鼠皮下移植骨肉瘤的光动力效应,并对两者进行比较,为筛选光动力疗法(PDT)治疗骨肉瘤的新药提供实验数据。方法:C3H小鼠60只,左侧腰骶部接种LM-8小鼠骨肉瘤细胞,待皮下瘤块直径约6～8mm时,随机分成空白对照组及PDT治疗组。治疗组分为PSD-007组(5mg.kg-1,用波长630nm激光照射)和BCPD-17组;BCPD-17组分设5和10mg.kg-12个剂量组,分别用波长630nm或670nm激光照射。小鼠尾静脉注射光敏剂后6h垂直瘤区行激光照射,功率密度200mW.cm-2、能量密度240J.cm-2、照射时间20min、光斑1.0cm。照射7d后测量肿瘤大小,取瘤体称重计算肿瘤抑制率,并行病理检查。结果:病理组织学观察,对照组小鼠骨肉瘤细胞大而深染,核异型明显;PDT治疗组肿瘤细胞出现坏死,细胞内空泡变性,核固缩,BCPD-17组更明显。PDT治疗组小鼠骨肉瘤的肿瘤质量及体积均明显减少,与对照组比较差异有统计学意义(P<0.01)。在670nm激光照射后,BCPD-17各组的肿瘤抑制率均高于PSD-007组,组间比较差异有统计学意义(P<0.01)。结论:光敏剂BCPD-17和PSD-007对小鼠LM-8骨肉瘤细胞有明确的抑制作用,光敏剂BCPD-17光动力效果更显著。Objective To observe and compare the photochemotherapeutic effects of BCPD-17（hematoporphyrin derivative） and PSD-007 on osteosarcoma LM-8 transplanted in mice and to explore a novel photosenitizer for photodynamic therapy of osteosarcoma Methods The tumor model was made by subcutaneous injection of osteosarcoma LM-8 on C3H mice.When the tumors reached about 6-8 mm in diameter,the tested mice were randomly divided into control group,PSD-007 group（5 mg·kg-1）,BCPD-17 groups（5 and 10 mg·kg-1）.After received intravenous injection of 5 or 10 mg·kg-1 of PSD-007 and BCPD-17,respectively,the tumors were irradiated vertically with 630 nm or 670 nm laser（power density 200 mW·cm-2,energy density 240 J·cm-2） for 20 min.The size of osteosarcoma was measured 7 d after treatment.And all the tumors were extirpated,weighted and taken for pathological examination.The inhibitory rate of tumor was calculated.Results The results of pathohistological observation showed that in control group the size of mouse osteosarcoma was big and hyperchromatic with obvious nuclear atypia;in PDT treatment group,the turmor cells presented apoptosis,vacuolar degeneration and karyopycnosis,especially in BCPD-17 group.Compared with control group,the volumes and the weights of the tumors in PDT treatment groups were obviously decreased,there were significant differences（P〈0.01）.Compared with PSD-007 group,the inhibitory rates of tumor in BCPD-17 groups irradiated by the laser with wavelength 670 nm were increased,there were significant differences（P〈0.01）.Conclusion Both of the photosenitizers BCPD-17 and PSD-007 could obviously inhibit the growth of the tumors.Photosenitizer BCPD-17 has more anti-tumor effect.

关 键 词：光动力疗法 LM-8骨肉瘤 BCPD-17 PSD-007 肿瘤抑制率 

分 类 号：R733.6[医药卫生—肿瘤] R738.1[医药卫生—临床医学]