幼鼠和老龄鼠尾胶原体外纤维化的形态学比较  

Morphological comparison of in vitro fibrillogenesis of tail collagen between young and aged rats

作　　者：刘国民[1] 孙红辉[1] 张艳秋[2] 张永利[3] 孔宁[4] 

机构地区：[1]吉林大学第二医院骨科,吉林长春130041 [2]吉林大学第二医院手术室,吉林长春130041 [3]吉林大学第二医院妇产科,吉林长春130041 [4]吉林大学药学院医用生物材料教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2012年第4期705-708,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅国际合作项目资助课题(20100417)

摘　　要：目的:通过观察幼鼠和老龄鼠尾胶原体外的纤维化过程以及幼鼠、老龄鼠腰椎松质骨胶原纤维形态学的变化,探讨年龄对胶原纤维化的影响。方法:取2周、20月的鼠尾肌腱各5g,分别利用稀醋酸进行胶原的提取,提取出的胶原低温冻干。取2周龄和20月龄的鼠尾胶原各15mg,分成3组,每组5mg,在4℃下分别熔解于10mmol.L-1醋酸溶液中;将钛金属螺钉浸润到醋酸溶液中,加入缓冲液,37℃下进行胶原的纤维化,分别在2、4和8h3个时间点取出螺钉,扫描电镜下观测螺钉表面的胶原形态。取2周、20月鼠各10只,处死后取出L3椎体,制成规则试件,反复生理盐水冲洗后,脱水,进行喷金后,行扫描电镜观察。结果:纤维化2h,2周龄及20月龄组鼠尾胶原纤维均未在螺钉表面微纤维化。纤维化4h,20月龄鼠尾胶原原纤维在螺钉的表面聚集成形态不规则的团块状胶原聚合物,未见胶原纤维形成;2周龄鼠尾胶原在螺钉的表面微纤维化成宽为0.07～0.20μm,纵横交错的胶原纤维。纤维化8h,20月龄鼠尾胶原在螺钉的表面聚集成原形或其他形态大小不等的胶原聚合物,未见胶原纤维形成;2周龄鼠尾胶原原纤维进一步纤维化,形成宽0.65～1.35μm的胶原纤维。骨小梁扫描电镜下显示:2周龄鼠骨胶原交联成粗细均匀的胶原纤维,纤维排列紧密靠拢,取向一致;20月龄鼠骨小梁胶原纤丝粗细不等,排列松散、稀疏、紊乱,其间联接相临的较粗纤丝的更细的胶原纤丝的取向杂乱无章。结论:随着年龄的增长,Ⅰ型胶原纤维化能力逐渐降低,导致骨胶原纤维形态的改变,矿物质沉积的异常,这可能是老年骨质疏松、骨折危险性增加的非常重要原因之一。Objective Through inspecting in vitro fibrillogenesis of the young and the old rat tail tendon collagen,as well as comparing the morphological changes of the lumbar cancellous bone collagen,to study the influence of age in fibrillogenesis.Methods The tail tendon（5 g） of 2-week and 20-month rats,was obtained,and the tail collagen was extracted by the dilute acetic acid.The extracted collagen was freezed-dried in low temperature.15 mg tail collagen were take from 2-month-old and 20-month-old rats.Each wss divided into three groups,5 mg in each group,and the collagen was melted in 10 mmol·L-1 acetic acid solution at 4℃,the titanium metal screws were infiltrated into the solution.After adding buffer the fibrillogenesis was taken in 37℃.The screws were picked up separately at 2,4 and 8 h,and the morphological changes of collagen of the surface of the screws were observed under scanning electron microscope（SEM）.10 2-week rats and 10 20-month rats were sacrificed to remove the L3 vertebral body,the regular specimen were made.The specimens were repeatedly washed by using physiological saline,dehydrated,and sprayed gold,and then observed under SEM.Results The tail collagen of rats in two groups did not take fibrillogenesis in the screw surface at 2 h.At 4 h,the tail collagen of 20-month-old rats was gathered into the irregular and massive collagen polymer,no collagen fibers wre formatted in surface of the screw.The tail collagen of 2-week-old rats turned into 0.07-0.20 μm collagenous fibres.At 8 h,the tail collagen of 20-month-old rats in the surface of the screw polymerized into the different size collagen polymers,no formation of collagen fibers.The tail collagen of 2-week-old rats formated 0.65-1.35 μm collagen fibers.The SEM results showed that the collagen of 2-week rat trabecular bone cross-linked into uniform collagen fibers.The fibers were closely arranged,and the orientation was consistent.The widths of the collagen fibrils of 20-month-old rat trabecular bone were not consistent.The arrangement of

关 键 词：幼鼠 老龄鼠 胶原体外纤维化 

分 类 号：R-332[医药卫生] R322.7