HLA-DRB1基因多态性与高血压肾病的关系  

Association between HLA-DRB1 gene polymorphisms and hypertensive nephropathy

作　　者：徐珊珊[1] 杨雪玲[2] 王琦 丁艳华[4] 宋艳玲 杨思嘉[1] 潘阳[1] 朱颖杰[1] 李波[1] 

机构地区：[1]吉林大学公共卫生学院流行病与卫生统计学教研室 [2]吉林大学公共卫生学院营养与食品卫生学教研室 [3]65301部队干休所 [4]吉林大学第一医院Ⅰ期药物临床实验病房 [5]吉林英联生物制药股份有限公司

出　　处：《吉林大学学报（医学版）》2012年第4期725-729,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30872174)

摘　　要：目的:探讨HLA-DRB1基因rs2308765和rs9269186 2个位点的单核苷酸多态性(SNPs)与高血压肾病的关系,为高血压肾病的遗传学机制研究提供理论依据。方法:采用病例-对照的研究方法,收集45例高血压肾病患者和52例对照为研究对象。应用聚合酶链反应-连接酶检测反应(PCR-LDR)技术进行SNPs突变检测。采用拟合优度χ2检验分析基因型分布是否符合Hardy-Weinberg平衡定律,病例组与对照组基因型和等位基因频数分布分析应用χ2检验。结果:rs2308765位点呈G/T二态性,本研究群体中出现G/G和G/T 2种基因型,病例组和对照组rs2308765位点的基因型和等位基因分布差异无统计学意义(P>0.05);rs9269186位点呈C/G二态性,本研究群体中出现了C/C、G/G和C/G 3种基因型,病例组和对照组的rs9269186位点的基因型分布和等位基因分布差异有统计学意义(P<0.05)。结论:rs2308765位点的SNPs可能与高血压肾病无关,而rs9269186位点的SNPs可能与高血压肾病有关。Objective To explore the association between single nucleotide polymorphisms（SNPs） at rs2308765 and rs9269186 sites of HLA-DRB1 gene and hypertensive nephropathy,and to provide theroretical basis for study on genetic mechanism of hypertensive nephropathy.Methods Case-control study method was used and 97 cases were collected in the study.PCR-LDR technique was used to detect the SNPs of HLA-DRB1 in 45 patients with hypertensive nephropathy and 52 controls.The goodness-of-fit χ2 test was used for Hardy-Weinberg equilibrium test in case and control groups.The differences of genotypic frequency and allelic frequency between case and control groups were test by χ2 test.Results There were G/T polymorphisms in rs2308765 sites,two genotypes G/G and G/T were found.The differences of genotype and allele distributions in rs2308765 between case and control were not significant（P〉0.05）.There were C/G polymorphisms in rs9269186 sites,three genotypes C/C G/G and C/G were found.The differences of genotype and allele distributions in rs9269186 between case and control groups were significant（P〈0.05）.Conclusion The correlation between SNPs at rs9269186 and hypertensive nephropathy is found,but not rs2308765.

关 键 词：高血压肾病 单核苷酸多态性 HLA-DRB1基因 

分 类 号：R394.3[医药卫生—医学遗传学]