兔肝金属硫蛋白提取工艺研究  

Study on Extraction Technology of Metallothionein from Rabbit Liver

作　　者：党蕊叶[1] 齐凡[2] 赵淑琳[1] 蒋志武[1] 王开锋[1] 权清转[1] 权启俊 冯成利[1] 

机构地区：[1]陕西省动物研究所,西安710032 [2]陕西省科学院,西安710043 [3]陕西同腾生物科技有限公司,陕西澄城715200

出　　处：《西北农业学报》2012年第6期22-25,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科学院重大项目(2010k-01-z4);陕西省农业攻关计划项目(2011K02-08)

摘　　要：研究提取液Tris-HCl的浓度、pH、加热温度和时间对兔肝金属硫蛋白提取的影响,并用正交试验优选出最适提取条件,为兔肝金属硫蛋白的研究提供一种新的提取技术。结果表明,Tris-HCl的浓度对金属硫蛋白的提取效果影响最大,pH和加热时间次之,温度对其影响最小。金属硫蛋白的合适提取条件为Tris-HCl的浓度0.01mol/L,pH 8.2,热处理温度80℃,加热时间8min。Zinc metallothionein was obtained by the induction of ZnSO4 from Belgian rabbit livers. Rabbit livers were added to the extraction solution, after being ground, heated and separated, an extraction solution for metallothionein was prepared. This paper studied the effect of concentration of Tris-HC1, pH value, the heating-up period and temperature on the extraction of metallothionein. Meanwhile, the orthogonal method was used to select the suitable conditions for metallothionein extraction, from rabbit livers. Test results showed that the concentration of Tris-HCl had the greatest effect on the extraction of metallothionein, followed by pH value, the heating-up period, and the heating temperature. The optimized conditions for metallothionein extraction are： Tris-HCl at 0.01 mol/L, pH value at 8.2, the heating temperature at 80 ℃and the heating-up for 8 minutes.

关 键 词：兔子肝脏 金属硫蛋白 提取 

分 类 号：S829.1[农业科学—畜牧学]