转Hoxc13基因绵羊的羊毛氨基酸组成及含硫量和含氮量分析  

Analysis of Amino Acid Contents,Sulfur Content and Nitrogen Content of Wool in Transgenic Sheep Carrying Hoxc13 Gene

作　　者：杨华[1] 周平[1] 杨永林[1] 刘守仁[1] 沈敏[1] 王新华[1] 姬勇[1] 

机构地区：[1]新疆农垦科学院兵团绵羊繁育生物技术重点实验室,新疆石河子832000

出　　处：《西北农业学报》2012年第5期37-40,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：转基因生物新品种培育重大专项(2008ZX08008-001;2009ZX08009-160B);新疆农垦科学院科技引导计划(YYD2010-8)

摘　　要：为评估转Hoxc13基因绵羊的羊毛化学特性,以中国美利奴(新疆军垦型)超细毛品系为对照组,转Hoxc13基因绵羊为试验组,应用水解法测定羊毛17种氨基酸,质量法测定羊毛含硫量,凯氏定氮法测量羊毛含氮量。结果表明,转Hoxc13基因绵羊的羊毛中天门冬氨酸、苏氨酸、丝氨酸、谷氨酸、脯氨酸、甘氨酸、丙氨酸、胱氨酸、缬氨酸、异亮氨酸、亮氨酸、酪氨酸、赖氨酸、组氨酸、苯丙氨酸和精氨酸的含量与对照组差异不显著(P>0.05),蛋氨酸的含量显著低于对照组(P<0.05),羊毛含硫量与对照组差异不显著(P>0.05),羊毛含氮量显著高于对照组(P<0.05)。以上结果说明,转Hoxc13基因绵羊的羊毛化学成分没有明显改变。To study chemical properties of wool in transgenic sheep carrying Hoxcl3 gene,the contents of 17 amino acids contents were assayed by hydrolysis method,sulfur content was assayed mass method and nitrogen content was assayed by Kjeldahl method in control group of Chinese Merino（Xinjiang Junken type）and test group of transgenic sheep carrying Hoxc13 gene. The results showed that contents of aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cystine, valine, isoleu cine, leucine, tyrosine, tysine, histidine, phenylalanine and arginine were not significantly different（P〉0.05） between control group and test group. The content of methionine in test group were significantly lower than that in control group（P〈0.05）. The sulfur content in test group was not significantly different from the control group（P〉0.05）. The nitrogen content in test group was significantly in- creased than in the control group（P〈0.05）. It was showed that chemical properties of the wool in transgenic sheep carrying Hoxc13 gene were not significantly changed.

关 键 词：转基因绵羊 Hoxc13基因 羊毛 氨基酸含量 含硫量 含氮量 

分 类 号：S826[农业科学—畜牧学]