液相色谱-串联质谱法检测水中微囊藻毒素LR和RR  

Determination of microcystin LR and RR in water by HPLC-MS/MS

作　　者：张艳[1] 张彩虹[1] 陈剑刚[1] 

机构地区：[1]广东省珠海市疾病预防控制中心,广东珠海519002

出　　处：《中国卫生检验杂志》2012年第7期1481-1483,共3页Chinese Journal of Health Laboratory Technology

基　　金：珠海市卫生局医学科研基金项目(2008041)

摘　　要：目的:建立水中微囊藻毒素LR和RR的液相色谱-串联质谱检测方法。方法:水样经C18固相萃取小柱富集,甲醇洗脱,浓缩后,经XTerra MS C18(2.1×150 mm,5μm)柱进行色谱分离,采用多反应监测(MRM)模式对微囊藻毒素进行检测,外标法定量。结果:微囊藻毒素LR和RR的检出限分别为0.002μg/L和0.001μg/L,其加标回收率分别为84.6%～90.6%和86.6%～91.2%,相对标准偏差小于5.0%。结论:该方法用水量少,简便、快速、准确,适用于水中微囊藻毒素LR和RR的检测。Objective：A HPLC-MS/MS method was developed for the determination of Microcystin LR and RR in water.Methods： Sample concentration and purification were performed using C18 solid phase extraction cartridges,the analyte was eluted by methanol.The separation was performed on a XTerra MS C18 column（2.1×150 mm,5 μm）.Multiple reaction monitoring（MRM） mode was applied in quantification of Microcystin LR and RR.Results： The limit of detection（LOD） was 0.002 μg/L and 0.001 μg/L for Microcystin LR and RR,respectively.The recoveries of microcystin LR and RR were 84.6%～90.6% and 86.6%～91.2%,respectively;with RSD less than 5.0%.Conclusion： This method is accurate and rapid,easy to operate for microcystin analysis in water.

关 键 词：微囊藻毒素LR 微囊藻毒素RR 水 液相色谱 质谱 

分 类 号：O657.63[理学—分析化学]