UPLC法测定保健品中五种人参皂苷的含量  

Determination of five ginsenosides in health products by UPLC

作　　者：郭健[1] 李敏[1] 高岩[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《中国卫生检验杂志》2012年第7期1507-1509,共3页Chinese Journal of Health Laboratory Technology

基　　金：卫生安全评价重点实验室(2007403016)

摘　　要：目的:建立人参类保健品中五种人参皂苷Rg1、Re、Rb1、Rc、Rd的超高效液相色谱分析方法。方法:样品经甲醇提取,大孔树脂柱净化,进行色谱分析。色谱条件:ACQUITY UPLC BEH C18色谱柱(2.1×50 mm,1.7μm);乙腈-水流动相体系;紫外检测,检测波长为203 nm;柱温30℃;流速为0.50 ml/min。结果:五种人参皂苷在20 min内得到良好分离,方法的线性范围为39.6μg/ml～500.0μg/ml,线性相关系数为0.9995～0.9997。最小检出量为0.8 ng～2.5 ng,回收率为78.6%～94.4%,相对标准偏差2.4%～6.4%。结论:本法具有简单、快速、准确、可靠的特点,能够满足日常分析检测的需要,为人参类保健食品的质量控制提供了简便方法。Objective：To develop a method for the determination of five ginsenosides（Rg1,Re,Rb1,Rc,Rd） in ginseng health products by UPLC.Methods： Samples were extracted by methanol,purified through macroporous adsorbents,and then the separation was performed on ACQUITY UPLC BEH C18 column（2.1×50 mm,1.7 μm）set at 30℃ for gradient elution,using the solution of acetonitrile and water as mobile phase at the flow rate of 0.50 ml/min and detected at 203 nm.Results： Five ginsenosides were well separated within 20 min.The calibration curves of ginsenosides showed good linearity in 39.6 μg/ml～500.0 μg/ml with correlation coefficients of 0.9995～0.9997.The detection limits of ginsenosides were 0.8 ng～ 2.5 ng.The recovery ranges were 78.6%～94.4%,and the RSDs were 2.4%～6.4%.Conclusion： The method is simple,rapid,sensitive and accurate,and suitable for the detection of five ginsenosides in health products.

关 键 词：超高效液相色谱法 人参保健品 人参皂苷 

分 类 号：O657.72[理学—分析化学]