四种梅毒检测方法的临床评价  

Clinical evaluation of four syphilis detection methods

作　　者：杨惠元[1] 

机构地区：[1]江苏省昆山市巴城人民医院检验科,江苏昆山215300

出　　处：《中国卫生检验杂志》2012年第7期1716-1717,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:通过酶联免疫吸附试验(ELISA)、快速血浆反应素试验(RPR)、胶体金法和梅毒螺旋体颗粒凝集试验(TPPA)四种方法对梅毒的检测,评价四种检测方法的敏感性和特异性。方法:对1215例病例,同时应用ELISA法、RPR法、胶体金法,若有阳性则用TPPA法确认检测,比较四种方法检测结果的相符率。结果:1215例标本中ELISA法阳性11例,RPR法阳性6例,胶体金法阳性13例,TPPA法阳性11例。结论:ELISA法是初筛梅毒试验的理想方法,胶体金法假阳性较高,RPR法假阴性较高,要结合TPPA确诊试验和临床表现等情况。Objective：To evaluate the sensitivity and specificity of ELISA,reaction plasma reagin（RPR）,colloidal gold method and treponema pallidum particle agglutination in syphilis detection.Methods： 1215 cases were detected with ELISA,RPR and colloidal gold method,and the positive cases were then confirmed by TPPA to compare the consistency of the four methods.Results： 11 cases were positive by ELISA,6 cases were positive by RPR,13 cases were positive by colloidal gold method and11 cases were positive by TPPA.Conclusion： ELISA was the optimal method for syphilis primary screening.Colloidal gold method had high false positive rate and RPR had high false negative rate,so the two methods need to combine with TPPA and clinical manifestation for accurate results in syphilis detection.

关 键 词：ELISA RPR 胶体金 TPPA 梅毒 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]