ADAM28反义核酸治疗牙根发育不良疾病的实验研究  

The study for treating congenital hypoplasia of tooth root used by ADAM28 AS-ODN

作　　者：赵征[1] 黄征难[1] 刘洪臣[2] 

机构地区：[1]解放军第401医院口腔科,山东青岛266071 [2]解放军总医院口腔科,北京100853

出　　处：《临床口腔医学杂志》2012年第8期471-474,共4页Journal of Clinical Stomatology

基　　金：中国博士后科学基金特别资助课题(201003774)

摘　　要：目的:探讨金属蛋白酶解离素28(ADAM28)反义核酸(AS-ODN)治疗牙根发育不良疾病(CHTR)的可行性和效果。方法:应用反义核酸技术、基因重组、动物实验和酶联免疫吸附测定(ELISA)法检测ADAM28 AS-ODN、S-ODN和真核质粒对CHTR的不同效应。结果:用药后AS-ODN组患牙松动度明显减轻,牙周袋深度显著变浅,COL-Ⅱ、IL-1β、PGE2水平均显著下降,呈现炎症愈合趋势;S-ODN组和真核质粒组各项检测结果正相反,炎症呈显著上升趋势。结论:应用ADAM28 AS-ODN(200μmol/L)可有效治疗牙根发育不良疾病,但最佳药物浓度和用药时间还需进一步探讨。Objective：To investigate the feasibility and effect of treating CHTR used by ADAM28 AS-ODN. nethod：Antisense oligonucleotides technique,gene recombination,animal experiment and ELISA were used to detect the different effects of ADAM28 AS-ODN, S-ODN and eukaryotic plasmid on CHTR. Result： After medication in AS-ODN group,mobility of tooth lessened obviously, depth of pocket shallowed notably,the levels of COL-Ⅱ , IL-1β and PGE2 lowered significantly which showed inflammation healing trend. Every detection result as above mentioned displayed opposite in S-ODN group and eukaryotic plasmid group, and inflammation was rising strikingly. Conclusion： The use of ADAM28 AS-ODN （200 μmol/L） could effectively treat CHTR but optimal drug concentration and medication time need further mveshgatlon.

关 键 词：金属蛋白酶解离素28 反义核酸 牙根发育不良 

分 类 号：R780.2[医药卫生—口腔医学]