干姜与附子配伍前后对干姜中4种姜辣素成分含量的影响  

Effect of Zingiber offiicinale and Aconitum cainichaeli before and after compatibility on contents of four gingerols

作　　者：彭稳稳[1] 李俊松[1,2] 李文[3] 蔡宝昌[2] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]江苏省中药炮制重点实验室,江苏南京210029 [3]南京市中医院,江苏南京210001

出　　处：《中国中药杂志》2012年第14期2076-2078,共3页China Journal of Chinese Materia Medica

摘　　要：目的:以干姜-附子药对为研究对象,采用HPLC测定干姜单煎液及不同配伍比例(1∶2,1∶1,1∶0.5)干姜与附子合煎液中6-姜酚、8-姜酚、6-姜烯酚、10-姜酚4种姜辣素类成分,探讨两药配伍使用可增效的作用机制。方法:Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流动相0.1%醋酸水溶液(A)-乙腈(B),梯度洗脱,0～30 min,40%～90%B;30～35 min,90%～40%B。流速1.0 mL.min-1,检测波长275 nm,柱温30℃。结果:4种姜辣素类成分均达到基线分离,线性关系良好(r>0.999),加样回收率为100.9%～103.5%,RSD<3.0%。与干姜单煎液相比,干姜附子合煎液中姜辣素类成分含量均呈上升趋势,且上升幅度与配伍附子量呈正比。结论:干姜与附子配伍可促进干姜中姜辣素类成分的溶出,从成分角度阐明干姜与附子配伍增效机制。Objective： To discuss the synergistic mechanism of compatible use of two medicinal herbs, Zingiber offiicinale and Aconitum cainichaeli, by determining single decoction of Z. offiicinale and four gingerols （6-gingerol, 8-gingerol, 6-shogaol, 10-gin- gerol） contained in compound decoction of Z. offiicinale and A. cainichaeli of different compatibility ratio using HPLC. Method ： Kro- masil-C18 column （4. 6 mm ×250 mm, 5 μm） was adopted. The mobile phase was acetonitrile （B） and 0. t% aqueous acetic acid （A） for gradient elution （0-30 min, 40%-90% B; 30-35 rain, 90% 40% B）. The flow rate was 1.0 mL· min^-1. The detection wavelength was set at 275 nm. The column temperature was 30 ℃. Result ： The four gingerols were in baseline separation, with a good linearity （r 〉 0. 999）, an average recovery of 100. 9% -103.5% and RSD 〈 3.0%. Compared with the single decoction of Z. offiicinale, the content of gingerols in the compound decoction of Z. offiicinale and A. cainichaeli was on the rise and in direct proportion with the increase in the volume of A. cainichaeli. Conclusion ： The synergistic mechanism of the compatibility of Z. offiicinale and A. cainichaeli can be proved with the increased release of gingerols from Z. offiicinale.

关 键 词：干姜 附子 配伍 姜辣素 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]