酶解辅助提取广藿香挥发油成分分析与抗肿瘤活性初探  

Extraction and Analysis of the Essential Oil in Pogostemon cablin by Enzymatic Hydrolysis and Inhibitory Activity Against Hela Cell Proliferation

作　　者：于敬[1] 齐悦[1] 罗刚[1] 段宏泉[1,2] 周晶[1] 

机构地区：[1]天津市临床药物关键技术重点实验室/天津医科大学药学院,天津300070 [2]天津医科大学基础医学研究中心,天津300070

出　　处：《中药材》2012年第5期796-799,共4页Journal of Chinese Medicinal Materials

基　　金：天津市科技攻关项目(06YFGPSH02900)

摘　　要：目的:优化广藿香挥发油的提取方法并考察其抗肿瘤活性。方法:在蒸馏法提取广藿香油之前进行酶解处理,通过正交试验研究酶的用量、酶解时间、温度以及pH对广藿香油提取率的影响,优选最佳工艺。采用气-质联用(GC-MS)测定广藿香挥发油的组成以及各成分的相对百分含量。通过MTT法测定广藿香油对Hela细胞增殖活性的抑制作用。结果:酶辅助提取广藿香油的最佳条件是:酶用量为生药重量的1%,在pH 4.5,45℃条件下酶解1 h;药典法、酶解法提取的总挥发油的提取率分别为2.2220、3.1360 mg/g;两种方法提取的广藿香油抑制He-la增殖细胞活性的IC50分别为:12.2±0.46、0.36±0.03μg/mL。结论:酶解法可提高广藿香挥发油的提取率,该法提取的广藿香油抑制Hela细胞增殖的活性强于药典法提取的广藿香油。Objective：To optimize the extraction method of essential oil in Pogostemon cablin and analyze its inhibitory activity a- gainst Hela cell proliferation. Methods：The Pogostemon cablin was treated by hemicellulase before steam distillation. The enzyme dos-age, treatment time, treatment temperature, pH were optimized through orthogonal experimental design. The components of essential oil were identified by gas chromatography-mass spectrometry （GC-MS）. Inhibitory activity of patchouli oil against Hela cell proliferation was determined by MT＇F method. Results：The optimum extraction process was as follows： pH 4. 5,temperature 45 ℃ the ratio of hemi-cellulase to Pogostemon cablin was 1% and enzymatic hydrolysis for 1.0 hour. Extraction ratio of the patchouli oil in steam distillation and hemicellulase extraction method was 2. 222 0 mg/g,3. 136 0 mg/g respectively. Patchouli oil could inhibit Hela cell proliferation. ICs0 of the patchouli oil in steam distillation and hemicellulase extraction method was 12. 2 ± 0. 46 μg/mL and 0. 36 ± 0.03 μg/mL re-spectively. Conclusion：In comparison with steam distillation method, extraction ratios of essential oil and the inhibitory activity against Hela cell proliferation can be increased by the hemicellulase extraction method.

关 键 词：广藿香 挥发油 半纤维素酶 GC-MS IC50 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]