乳腺癌患者外周血CK19 mRNA表达浓度监测的意义  

The significance of peripheral blood CK19 mRNA expression in breast cancer patients

作　　者：赵亚新[1] 刘洁凡[2] 孙忠敏[3] 江明华[4] 郑志强[1] 

机构地区：[1]浙江省温州医学院附属第二医院普外科,325000 [2]温州医学院附属第一医院全科医学科 [3]温州医学院附属第二医院药剂科 [4]温州医学院附属第二医院检验科

出　　处：《中华普通外科杂志》2012年第8期656-659,共4页Chinese Journal of General Surgery

基　　金：浙江省自然基金项目（Y2080476）,浙江省中医药局基金项目（2009C520014）,温州市科技局项目（Y20070121）

摘　　要：目的探讨监测乳腺癌患者外周血中CK19mRNA表达浓度的临床意义。方法用实时定量PCR法测定137例乳腺癌患者术前、术后第1天、术后第7天、术后1个月、术后3个月、术后6个月、术后12个月、术后18个月及术后24个月等各时间点的外周血中CK19mRNA的表达，分析CK19浓度变化与乳腺癌复发转移之间的关系。结果①术前、术后第1天、术后第7天3个时间点乳腺癌患者外周血的CK19mRNA的浓度无明显变化（P〉0．05）；术后1个月CK19mRNA水平较术前、术后第1天及术后第7天相比均有明显下降（P〈0．05）。未复发组的外周血中CK19mRNA浓度持续维持在低水平，而复发转移组的CK19mRNA表达浓度在下降后再次升高（P〈0．01）。②CK19mRNA的表达与月经状态、雌激素受体、孕激素受体、HER2和Ki67表达无明确相关（P〉0．05）；与肿瘤大小、肿瘤组织学分级、病理类型及淋巴结转移等指标均有明显相关性（P〈0．01）。结论乳腺癌患者外周血CK19mRNA的表达与临床病理高危因素指标相关，复发转移时其浓度升高，可能成为乳腺癌患者复发转移的指标。Objective To evaluate the significance of CK19 mRNA monitoring in peripheral blood of breast cancer patients. Methods Peripheral blood samples were collected from 137 breast cancer patients preoperatively, one day post-operation, 7 days, 1,3,6,12,18, and 24 months after operation. RT- PCR was used to detect CK19 mRNA expression. The relationships were analyzed between CK19 mRNA expression and treatment result, between CK19 mRNA expression and elinicopathological parameters. Results There was no significant difference between the level of CK19 mRNA as tested preoperatively, and 1,7 days postoperatively（P 〉0. 05）. From one month and thereafter, CK 19 mRNA decreased significantly when compared with that immediate perioperatives （ P 〈 0.05 ）. Postoperative peripheral CK19 mRNA expression increased in those found with recurrent or metastasized tumors （ P 〈 O. 01 ）. CK19 mRNA expression does not correlate with patient＇s menstrual status, estrogen receptor expression, progesterone receptor expression, HER-2 expression and Ki67 proliferation expression （ P 〉 0. 05 ）. But there was statistically significant correlation between CK19 mRNA expression and tumour size, histology grading, pathological type, lymph node metastasis （ P 〈 0. 01 ）. Conclusions In breast cancer patients peripheral blood CK19 mRNA expression is correlated with risk clinicopathological parameters, and increases in patients with postoperative recurrence and tumor metastasis.

关 键 词：乳腺肿瘤 角蛋白质类 预后 复发 

分 类 号：R737.9[医药卫生—肿瘤]