人疱疹病毒6型感染与神经胶质瘤关系的初步研究  

Preliminary study on the relationship between human herpesvirus-6 infection and glioma

作　　者：张春[1] 茌静[2] 顾斌[1] 郭丹丹[2] 张国锋[1] 周锋[2] 魏栋[1] 姚堃[2] 胡卫星[1] 

机构地区：[1]南京医科大学第一附属医院神经外科,江苏南京210029 [2]南京医科大学微生物与免疫学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2012年第8期1052-1056,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30972784);江苏省医学重点人才工程(135工程)资助(RC2002075)

摘　　要：目的:研究人疱疹病毒6型(human herpesvirus-6,HHV-6)感染与神经胶质瘤的关系。方法:巢式PCR法检测40例神经胶质瘤样本和13例正常脑组织样本中HHV-6、人巨细胞病毒(human cytomegalovirus,HCMV)、HHV-7 DNA序列。免疫组化染色法检测神经胶质瘤样本和正常脑组织样本中HHV-6、HCMV、HHV-7抗原的表达。结果:巢式PCR法检测结果显示,神经胶质瘤组织HHV-6 DNA阳性率为42.5%,正常脑组织HHV-6 DNA阳性率为7.7%(P=0.020),神经胶质瘤组织HCMV、HHV-7DNA阳性率分别为20.0%和5.0%,正常脑组织未检测到HCMV和HHV-7 DNA(P值分别为0.087和0.566)。进一步用免疫组化染色法检测HHV-6早期抗原p41的表达,神经胶质瘤组织阳性率为27.5%,正常脑组织未见阳性表达(P=0.030)。同时检测HHV-6晚期抗原gp116/64/54的表达,神经胶质瘤组织阳性率为32.5%,正常脑组织未见阳性表达(P=0.014)。用HCMV pp65抗体进行免疫组化染色后发现其在神经胶质瘤中阳性率为12.5%,正常脑组织未见阳性表达(P=0.229)。HHV-7 pp85抗原在神经胶质瘤样本和正常脑组织样本中均未检出阳性表达。结论:根据PCR和免疫组化的检测结果,HHV-6感染在神经胶质瘤和正常脑组织中具有显著性差异,HHV-6感染在神经胶质瘤的病因和发展过程中起了一定作用。Objective： To study the relationship between human herpesvirus（HHV）-6 infection and glioma.Methods：HHV-6,human cytomegalovirus（HCMV） and HHV-7 DNA in glioma samples（n = 40） and control samples（n = 13） were detected by nested PCR.The expression of HHV-6,HCMV and HHV-7 was detected by immunohistochemistry（IHC）.Results：HHV-6 DNA was positive in 17/40（42.5%） of glioma samples and 1/13（7.7%） of normal brain samples（P = 0.020）,while the positive rates of HCMV and HHV-7 DNA were 20.0% and 5.0% in glioma,respectively.HCMV and HHV-7 DNA hadn’t been found in normal brain samples（P = 0.087 and 0.566 respectively）.HHV-6 late antigen p41 was expressed in 11/40（27.5%） of tumors and 0 of the controls（P = 0.030）,while HHV-6 early antigen gp116/64/54 was expressed in 13/40（32.5%） of tumors and 0 of the controls（P = 0.014）.HCMV pp65 antigen was expressed in 5/40（12.5%） of glioma samples and 0/13 of the normal brain controls（P = 0.229）.HHV-7 pp85 antigen hadn’t been found in tumors and normal brain samples.Conclusion：Based on the results of nested PCR and immunohistochemistry,significant difference of HHV-6 infection between glioma and normal brain tissue was confirmed.HHV-6 infection may play a role on the pathagenesis and progression of glioma.

关 键 词：人疱疹病毒6型 神经胶质瘤 巢式PCR 免疫组化 

分 类 号：R739.41[医药卫生—肿瘤]