HPLC同时测定豆制品中常见的3种食品添加剂  

Determination of three food additive in soy products by high-performance liquid chromatography

作　　者：朱建丰[1] 

机构地区：[1]江阴市疾病预防控制中心,江苏江阴214400

出　　处：《食品与机械》2012年第4期87-88,99,共3页Food and Machinery

摘　　要：采用反相高效液相色谱法快速、准确测定豆制品中的苯甲酸、山梨酸和糖精钠。3种添加剂在Zorbax sb-C18色谱柱上分离,甲醇-乙酸铵溶液(5∶95,V∶V)为流动相洗脱,流速1.0mL/min,15min内实现良好分离。方法检出限为0.5mg/kg,变异系数为0.67%～1.23%,回收率在97.7%～99.7%。该方法简便准确,适合豆制品的日常检测。To develop a rapid and accurate method for determination of benzoic acid, sorbic acid and Saccharin Sodium in soy products by RP- HPLC, with the chromatographic column of Zorbax sb-C18, the mo- bile phase consisted of methanol ammonium acetate （5 ： 95 ,V： V） at the flow rate of 1. 0 mL/min ,The 3 compounds were analyzed in 15 rain. The detection limit was 0. 1 mg/kg, relative SD 0. 67%- 1.23% and recovery 97.7%-99.7%. This method is simple and ac- curate, can be applied to the determination of soy products.

关 键 词：豆制品 苯甲酸 山梨酸 糖精钠 高效液相色谱 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]