R-1-苯基乙基氨基甲酸酯-β-环糊精键合手性固定相的制备与应用研究  

Preparation and Application of R-1-Phenylethylcarbamoylated β-CD Bonded Chiral Stationary Phase

作　　者：郭丽珍[1] 余光艳[1] 望丹妮[1] 谢飞云[1] 李杭燕[1] 林杰[1] 翁文[1] 

机构地区：[1]漳州师范学院化学与环境科学系,福建漳州363000

出　　处：《福建分析测试》2012年第4期1-6,共6页Fujian Analysis & Testing

基　　金：国家自然科学基金资助项目(20705031);福建省高校新世纪优秀人才支持计划资助项目(JK2011030)

摘　　要：利用R-1-苯基乙基异氰酸酯对β-环糊精键合固定相进行衍生,合成了R-1-苯基乙基氨基甲酸酯-β-环糊精手性固定相,填充后在反相条件下考察其对氢化安息香、安息香和α-苯乙醇的手性拆分,探讨了流动相中乙腈含量、缓冲盐类型等对手性拆分的影响。氢化安息香获得了基线分离,分离因子可达1.214,安息香得到了部分分离,α-苯乙醇未能拆开。结合线性溶剂强度(LSS)模型和计量置换理论(SDM-R)对色谱保留机理进行了探讨,认为水分子和乙腈分子一起参与了溶质的置换。R-1-Phenylethylearbamoylated β -CD bonded chiral stationary phase （CSP） was prepared from R-1-phenylethyl isocyanate and β -cyclodextrin bonded CSP. The obtained CSP was packed into stainless steel column by slurry method. Then enantioseparations of hydrobenzoin, benzoin and α -phenethyl alcohol were evaluated under the reverse mode. The effects of acetonitrile amount and buffer type on the retention and enantioseparation were investigated. Hydrobenzoin obtained baseline separation, and the separation factor could reach 1.214. Benzoin obtained partial enantioseparation, and α -phenethyl alcohol could not be separated wholly. Chromatographic retention mechanism was studied further with linear solvent strength （LSS） model and stoichiometric displacement method for retention （SDM-R）. Water molecules and acetonitrile molecules were considered to participate in the displacing process of the solute.

关 键 词：手性固定相 Β-环糊精 R-1-苯基乙基异氰酸酯 氢化安息香 色谱保留机理 

分 类 号：O656.4[理学—分析化学]