青蒿琥酯治疗对斯氏狸殖吸虫感染大鼠血清IL-2和IL-4含量的影响  

Effects of artesunate on IL-2 and IL-4 in the sera of rats infected with Pagumogonimus skrjabini

作　　者：李鹏华[1] 畅灵丽[1] 黄玉清[2] 余俊萍[1] 张金平[1] 王光西[1] 

机构地区：[1]泸州医学院病原生物学教研室,泸州646000 [2]泸州医学院卫生科,泸州646000

出　　处：《国际医学寄生虫病杂志》2012年第4期207-210,共4页International JOurnal of Medical Parasitic Diseases

基　　金：基金项目：四川省卫生厅科研基金（100232）

摘　　要：目的对比观察青蒿琥酯（Art）和吡喹酮治疗斯氏狸殖吸虫感染大鼠后，大鼠免疫转归情况，了解青蒿琥酯是否有提高斯氏狸殖吸虫感染大鼠免疫力的作用。方法将36只清洁级雄性Wistar大鼠随机分成6组，正常对照组（A组），感染未治疗组（B组），青蒿琥酯小剂量组（c组）、中剂量组（D组）、大剂量组（E组），吡喹酮组（F组）。感染鼠每只腹腔注射25个斯氏狸殖吸虫囊蚴，感染后30d开始用药治疗。C、D、E分别给予Art50mg／（kg·d）×7d，100mg／（kg·d）×7d，150mg／（kg·d）×7d口服；F组给予吡喹酮150mg／kg，隔日给药一次，共3次；B组给予同体积生理盐水。于用药后30d，摘取大鼠眼球取血，分离血清，取脾称重，计算脾脏指数。用ELISA测定大鼠血清中的细胞因子白细胞介素-2（IL-2）和白细胞介素4（IL-4）的含量。结果A组大鼠脾脏指数、IL-2、IL_4含量依次为2．7333±0．16330、（28．3333±1．05409）pg／ml、（4．1190±0．44537）pc,／ml；B组大鼠脾脏指数、IL-2、IL-4含量依次为3．8333±0．25033、（19．5000±0．76376）pg／ml、（41．9293±0．80702）pg／ml，与A组相比，有统计学意义差异（t=9．20、16．62、100．48，P均〈0．01）。c、D、F组与B组比较，脾脏指数、IL-2、IL-4有统计学意义差异（f=6．00、7．50、8．63，10．70、18．54、11．51，112．54、113．21、111．39，P均〈0．01），E组脾脏指数、IL-2与B组比较，无统计学意义差异（t=1．75，0．54，P均〉0．05）。结论中、小剂量的青蒿琥酯有提高斯氏狸殖吸虫感染大鼠免疫力的作用。Objective To observe the effects of artesunate（Art） on IL-2 and IL-4 in the sera of rats infected with Pagumogonimus skrjabini. Methods 36 male Wistar rats were selected for experiment. 30 wistar rats were intra-abdominally injected with 25 metacercariae and randomly divided into 5 groups （ group B as untreated infective group）. 6 normal Wistar rats were randomly selected as control group （A group）. 30 days after infection, the rats in group C, D, E were intragastrically administrated with Art with the dose of 50 mg/ （kg- d）, 100 mg/（kg ~ d） and 150 mg/（kg ~ d） for consecutive 7 days, the rats in group F were intragastrically administration with praziquantel 150 mg/kg, qod, 3 times. The rats in group B were administered with equal volume of normal saline. Orbital blood was collected for qualification of IL-2 and IL-4 which were detected in serum by ELISA. The spleen were weighed for calculation of spleen index. Results The spleen index, IL-2, IL-4 of the rats of group A were 2.7333 ±0. 16330, （28. 3333 ± 1. 05409） pg/ml and （4. 1190±0.44537） pg/ml . The spleen index, IL-2 and IL-4 of the rats of group B were 3. 8333 ±0. 25033, （19. 5000 ±0. 76376） pg/ml and （41. 9293± 0. 80702 ） pg/ml. There were significant difference between group A and group B（t =9.20,16.62,100.48 respectively,P 〈0.01 ）. Comparing with group B, the spleen index, IL-2 and IL-4 of the rats of group C, D and F were significantly different （ t = 6.00, 7.50, 8.63, 10.70,18.54,11.51,112.54,113.21,111.39 respectively, P 〈 0. 01 ）. There were no significant difference between group B and E （ t = 1.75,0. 54,P 〉 0. 05） about the spleen index and IL-2. Conclusion Low andmiddle doses Art exhibit the effect of enhancing the immunity of the rats infected with P. skrjabini.

关 键 词：大鼠 斯氏狸殖吸虫 青蒿琥酯 白细胞介素-2 白细胞介素-4 

分 类 号：R-332[医药卫生]