旋毛虫新生幼虫可溶性抗原的分析  

The analysis of worm soluble antigens of Trichinella spiralis newborn larvae at different stages

作　　者：马鸣旺[1] 赵雪[2] 申丽洁[3] 

机构地区：[1]山西医科大学汾阳学院检验系,汾阳032200 [2]云南省疾病预防控制中心,昆明650034 [3]大理学院寄生虫学教研室,大理671000

出　　处：《国际医学寄生虫病杂志》2012年第4期211-214,共4页International JOurnal of Medical Parasitic Diseases

摘　　要：目的初步分析云南大理旋毛虫不同时龄新生幼虫可溶性抗原的蛋白组分及其免疫反应性。方法以十二烷基硫酸钠一聚丙烯酰胺凝胶电泳（SDS—PAGE）和蛋白印迹（Westernblot）对旋毛虫不同时龄新生幼虫可溶性抗原进行蛋白组分及其免疫反应性分析。结果SDS．PAGE结果显示：24h旋毛虫新生幼虫可溶性抗原显示36条蛋白染色条带，相对分子质量（幔）范围在160000—10000之间，其中主带8条，帆分别为73000、64000、58000、54000、46000、40000、34000、25000；48h旋毛虫新生幼虫可溶性抗原显示40条蛋白染色带，肘，范围在172000～10000之间，其中主带17条，恤分别为120000、102000、89000787000779000、74000、72000、64000、58000、54000、46000、40000、34000、32000、25000、18000、10000。Westernblot结果：以旋毛虫感染大鼠血清检测，24h旋毛虫新生幼虫可溶性抗原出现7条反应条带，恤分别为89000、78000、64000、58000、40000、34000、25000；48h新生幼虫可溶性抗原出现14条反应条带，M1分别为89000、87000、79000、74000、72000、64000、58000、40000、38000、34000、32000、25000、18000、10000。而正常大鼠血清在相应位置均无特异性反应条带出现。结论云南大理旋毛虫24、48h新生幼虫可溶性抗原存在7个共同蛋白组分，其中48h新生幼虫虫体可溶性抗原中存在24h新生幼虫可溶性抗原没有的5个蛋白组分，新生幼虫虫体可溶性抗原的蛋白组分随时龄的增大而增多。旋毛虫感染大鼠血清均可识别24、48h新生幼虫可溶性抗原中6个蛋白组分，说明这些蛋白组分具有较强的免疫反应性。Objective To analyze the protein components and the immunoreactivity of Trichinella spiralis new born larva （NBL） soluble antigens at different stages in Dali, Yunnan. Methods The sodium dodecyl sulfate-polyaerylamide gel electrophoresis （SDS-PAGE） was used to analyze protein components of the worm soluble antigens of NBL at different stages, and the Western-blot was used to analyze the immunoreaetivity of the worm soluble antigens of NBL at different stages against T. spiralis infected rats. Results The results from SDS-PAGE analysis displayed that the worm soluble antigens of T. spiralis NBL on 24 h showed 36 protein bands with the range between Mr160 000 and 10 000, among which the 8 main bands were Mr73 000, 64 000, 58 000, 54 000, 46 000, 40 000, 34 000 and 25 000, respectively. The worm soluble antigens of T. spiralis NBL on 48 h displayed that 40 proteins bands with the range between 172 000 and 10 000, among which the 17 main bands were 120 000, 102 000, 89 000, 87 000, 79 000, 74 000, 72 000, 64 000, 58 000, 54 000, 46 000, 40 000, 34 000, 32 000, 25 000, 18 000 and 10 000, respectively. The results of Western-blot showed that 7 protein bands of the worm soluble antigens from T. spiralis NBL on 24 h reacted with the serum of rat anti-T, spiralis NBL. Whereas, 14 protein bands of the worm soluble antigens from T. spiralis NBL on 48 h reacted with the serum of rat anti-T, spiralis NBL. Moreover, the negative serum had no cross-reactivity to corresponding proteins. Conclusion The worm soluble antigens of T. spiralis NBL on 24 h had 7 same protein components in comparison with NBL on 48 h. The protein components from the worm antigens of T. spiralis NBL increased during the development of the worm. Western-blot displayed thatthe serum of rat anti-T, spiralis NBL had stronger specific recognition to the 6 protein components from the worm soluble antigen of T. spiralis NBL both on 24 h and 48 h. Thus, these protein components had stronger immunoreactivity.

关 键 词：旋毛虫 不同时龄新生幼虫 虫体可溶性抗原 蛋白组分 免疫反应性 

分 类 号：R383.15[医药卫生—医学寄生虫学]