曝气生物滤池工艺脱氮性能及反硝化细菌群落结构特征研究  

Study on the Nitrogen Removal Performance and the Characteristics of Denitrification Bacterial Community Structure of Biological Aerated Filter

作　　者：彭晓兰[1] 刘聪[1] 陈吕军[1,2] 

机构地区：[1]清华大学环境学院,北京100084 [2]浙江清华长三角研究院生态环境研究所,浙江省水质科学与技术重点实验室,嘉兴314006

出　　处：《环境科学》2012年第8期2701-2708,共8页Environmental Science

基　　金：北京市城市节约用水办公室项目(041504015)

摘　　要：比较了两组不同填料组合的前置反硝化曝气生物滤池(biological aeration filter,BAF)脱氮工艺:陶粒-沸石BAF工艺(C-Z BAF)和沸石-陶粒BAF工艺(Z-C BAF),在不同C/N条件下的脱氮效果及滤料表面的反硝化细菌群落结构特征.结果表明,Z-C BAF对TN的平均去除效率高于C-Z BAF,且在低C/N条件下Z-C BAF的优势更加明显,当C/N为3.3时Z-C BAF的TN平均去除率比C-Z BAF高出20%.测定2种组合在不同C/N条件下的反硝化细菌群落结构发现,随着C/N的降低,2种组合反硝化细菌的物种丰度和多样性显著下降.低C/N条件下,Z-C BAF整个好氧滤池的nosZ基因优势T-RFs相对丰度>20%,而C-Z BAF好氧滤池顶端样品不能有效扩增到nosZ基因,可推断Z-C BAF的同步硝化反硝化功能优于C-Z BAF.在低C/N条件下Z-C BAF的脱氮效率明显高于C-Z BAF,因此Z-C BAF在低C/N且低浓度的有机废水处理方面更有潜力.The nitrogen removal performance and the denitrification bacterial community structure of two pre-denitrification biological aeration filter（BAF） processes with different media combinations,the ceramic-zeolite BAF process（C-Z BAF） and the zeolite-ceramic BAF process（Z-C BAF）,were compared at different C/N ratios.The results showed that the average TN removal efficiency of Z-C BAF was higher than that of C-Z BAF,and the advantage was more notable at low C/N ratio.When the C/N ratio decreased to 3.3,the average TN removal efficiency of Z-C BAF was 20% higher than that of C-Z BAF.Comparing the microbial community structure of the two combinations at different C/N ratios,it was found that the richness and diversity of denitrification bacterial community decreased significantly with the decrease of C/N ratio.At low C/N ratio,within the whole oxic filter of Z-C BAF,nosZ genetic T-RFs were detected with more than 20% relative abundance,whereas no nosZ genetic T-RFs were detected at the upper oxic filter of C-Z BAF by PCR amplification.The results indicated that the simultaneous nitrification and denitrification function of Z-C BAF was better than that of C-Z BAF.At low C/N ratio,the nitrogen removal efficiency of Z-C BAF was obviously higher than that of C-Z BAF.Thus,compared to C-Z BAF,Z-C BAF had greater potential on the treatment of wastewater with low organic concentration and low C/N ratio.

关 键 词：曝气生物滤池 前置反硝化脱氮工艺 C/N T—RFLP 反硝化细菌群落结构 

分 类 号：X172[环境科学与工程—环境科学] X703.1