多西环素对LPS诱导的BV-2细胞MHCII表达的抑制效应  

Doxycycline down-regulates MHC II expression induced by LPS in BV-2 microglia

作　　者：王普清[1] 孙圣刚[2] 

机构地区：[1]湖北医药学院附属襄阳医院,湖北襄阳441000 [2]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《中风与神经疾病杂志》2012年第7期606-609,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究多西环素对LPS诱导的BV-2细胞MHCII表达的影响及其分子机制。方法 BV-2细胞作为小胶质细胞的替代细胞。(1)按常规方法培养BV-2细胞;(2)分组及处理:①对照组:不加任何特殊处理;②脂多糖组:脂多糖(100ng/ml)与BV-2细胞共孵育24h;③多西环素预处理组(多西环素+LPS):BV-2细胞在6孔板分别与多西环素(3个浓度梯度:50μmol/L、100μmol/L、200μmol/L)预孵育30min,然后,细胞再用脂多糖(100ng/ml)处理24h;④多西环素对照组:用细胞免疫荧光共聚焦显微镜观察。(3)Westen Blot检测MHCII蛋白的表达。结果 (1)细胞免疫荧光显示,分别用3种浓度(50μmol/L、100μmol/L、200μmol/L)多西环素预处理后,BV-2细胞MHCII的表达减少,并有量-效关系;(2)Westen Blot显示,多西环素预处理后,BV-2细胞的MHCII蛋白表达减少,3种浓度梯度显示有量-效关系(P<0.01)。结论研究初步证实多西环素预处理能够抑制LPS诱导的BV-2小胶质细胞MHCII的表达。Objective To explore the inhibitory effects of Doxycycline(DC)on expression of MHCII in BV-2 microglia.Methods BV-2 was cultured as a substitute of microglias,which were divided into 3 groups:the control group(no any special handling),lipopolysaccharide(LPS)group(LPS+BV-2),and Doxycycline pre-treatment group(BV-2 was stimulated by LPS after co-incubation with Doxycycline at 3 concentration gradients for 30min).BV-2 microlia were detected with immunofluorescence confocal microscopy.The levels of MHCII were evaluated by Western Blot.Results Doxycycline pre-treatment could inhibite MHCII expression in BV-2 by immunofluorescence,with quantity-effect relation.Western blot displayed that Doxycycline pre-treatment blockaded MHCII expression(P<0.01).Conclutions Doxycycline inhibites MHCII expression in BV-2 microlia.

关 键 词：多西环素 MHCII BV-2细胞 

分 类 号：R742.5[医药卫生—神经病学与精神病学]