响应面分析法优化西兰花离体细胞系萝卜硫素提取工艺  

Optimization of extraction technique of sulforaphane from broccoli callus via by response surface methodology(RSM)

作　　者：杨海荣[1] 马绍英[1] 赵利敏[1] 杨柯[1] 张品南[1] 刘会杰[1] 戴彩虹[1] 付瑞军[1] 李胜[1] 

机构地区：[1]甘肃农业大学生命科学技术学院植物细胞工程研究室,甘肃兰州730070

出　　处：《食品工业科技》2012年第15期206-209,214,共5页Science and Technology of Food Industry

基　　金：教育部博士点新教师基金(20096202120008);甘肃农业大学SRTP(20110805)

摘　　要：以西兰花子叶愈伤组织为实验材料,利用响应面法对西兰花愈伤组织萝卜硫素的提取工艺进行优化。在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用四因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以愈伤组织中萝卜硫素提取率为响应值作响应面和等高线。在分析各个因素的显著性和交互作用后,得出浸提愈伤组织中萝卜硫素的最佳工艺条件为:温度25.7℃、固液比1:30(g/mL)、浸提时间2.8h、提取次数3次。在此条件下萝卜硫素产量实测值为0.0067%(理论值为0.0078%),说明采用响应面法优化得到的提取条件可靠。Using broccoli callus as experimental material,based on single factor tests,through Box-Benhnken central combination design and RSM were obtained the optimum extraction conditions of sulforaphane.The results showed that the optimum extraction conditions were the extraction temperature 25.7℃,the extraction time 2.8h,the liquid solid ratio 1：30（g/mL）,the extraction times 3.The test value was 0.0067% under the above conditions（theory value was 0.0078%）,that optimize the use of response surface method of the extraction conditions were reliable.

关 键 词：西兰花愈伤组织 萝卜硫素 提取 响应面分析 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]