不同竹种竹叶提取物制备及其抗氧化活性比较研究  

Preparation and comparative analysis of antioxidant activity of bamboo leaf extracts from different varieties

作　　者：王慧[1] 岳永德[1] 郭雪峰[1] 汤锋[1] 王进[1] 姚曦[1] 孙嘏[1] 

机构地区：[1]国际竹藤中心国家林业局竹藤科学与技术重点开放实验室,北京100102

出　　处：《安徽农业大学学报》2012年第4期540-544,共5页Journal of Anhui Agricultural University

基　　金："十二"五国家科技支撑计划项目(2012BAD23B03)资助

摘　　要：以毛竹、苦竹、香竹、箬竹、粉单竹、青杆竹和青皮竹7个竹种竹叶为材料,采用热回流法提取,大孔树脂法分离纯化,对得到竹叶提取物测定其得率和主要黄酮成分的含量,进而探讨竹叶提取物的抗氧化活性,为竹叶提取物在食品和化妆品领域的应用提供科学依据。结果表明,苦竹叶提取物得率最高,为3.60%～3.70%;香竹叶提取物中所含的荭草苷和异荭草苷量最高,为2.523 0%～2.532 6%,苦竹叶提取物中所含的牡荆苷量最高,为0.887 0%～0.889 6%,青杆竹叶提取物中所含的异牡荆苷量最高,为2.223 1%～2.226 1%;竹叶提取物具有较强的自由基清除作用;香竹表现的DPPH自由基清除能力最强,IC50值为30.50 mg·L-1;箬竹的超氧阴离子和羟自由基清除效果最强,IC50值分别为339.32 mg·L-1和324.87 mg·L-1。Phyllostachys heterocycla, Pleioblastus amaru, Bambusa tuldoides, Bambusa ehungii, Bambusa textilis, Chimonocalamus Hsueh and lndocalamus tessellates were used to extract bamboo-leaf-flavonoids by the heat reflux method and purified by AB-8 macroporous absorption resin. The extractions were separated and struc- turally identified by high-performance liquid chromatography （HPLC）. The bamboo leaf extracts were assayed based on DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, superoxide anion free radical scavenging capacity, and reducing power with TBHQ （tert-butyl hydroquinone） as the positive control. The results showed that the yield of extracts from Pleioblastus amarus was maximum, up to 3.60%-3.70%; the amount of orientin and isoorientin in Chimonocalamus Hsueh was maximum, up to 2.523 0%-2.532 6%; the amount of vitexin in Pleioblastus amarus was maximum, up to 0.887 0%-0.889 6%; the amount of isovitexin in Bambusa textilis was maximum, up to 2.223 1%-2.226 1%. Chimonocalamus Hsueh revealed the strongest scavenging ca- pacity against DPPH, and the IC50 value was 30.50 tug＇L-l; Indocalamus tessellates revealed the strongest scav- enging capacity against hydroxyl radical and superoxide anion free radical, and the IC50 values were 339.32 mg.L-1 and 324.87 mg＇L-t, respectively.

关 键 词：竹叶提取物 抗氧化活性 IC50值 

分 类 号：Q946.8[生物学—植物学]