乌司他丁对感染性脑水肿的干预作用及相关机制研究  

The treatment effects and possible mechanism of ulinastatin on infectious brain edema

作　　者：刘济滔[1] 周凯[1] 胡沥[1] 

机构地区：[1]泸州医学院附属医院急诊科,四川泸州646000

出　　处：《检验医学与临床》2012年第15期1855-1857,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨乌司他丁对感染性脑水肿的治疗作用以及对星形胶质细胞在感染性脑水肿中的保护作用。方法 55只按照颈内动脉注射脂多糖(150μg,0.15mL)的方法建立感染性脑水肿模型的大鼠,随机分为3组:乌司他丁处理组(U组,n=45);生理盐水对照组(S组,n=5);空白对照组(C组,n=5)。乌司他丁处理组又按照乌司他丁处理后6、12、24h分为3个亚组(n=15)。采用HE染色观察脑组织病理改变;干湿重法检测脑组织含水量;流式细胞术检测星形胶质细胞凋亡情况;胶质纤维酸性蛋白(GFAP)免疫组化技术观察星形胶质细胞活化情况。结果所有实验组大鼠HE染色可见脑组织弥漫性水肿;U组大鼠细胞含水量明显低于S组以及C组,差异有统计学意义(P<0.05),C组与S组间差异无统计学意义(P>0.05);各实验组星形胶质细胞均出现凋亡,在实验24h最为明显,U组与C组、S组比较,差异有统计学意义(P<0.05),各亚组间比较差异有统计学意义(P<0.05)。免疫组化染色可见U组、C组和S组均出现GFAP阳性表达细胞;U组与C组、S组比较,GFAP表达增强。结论乌司他丁在感染性脑水肿中具有明显的脑保护作用,该作用与乌司他丁抑制星形胶质细胞异常活化以及凋亡有关。Objective To investigate the treatment effects of ulinastatin on infectious brain edema and the pro tective effects of ulinastatin on astrocytes in infectious brain edema rats. Methods 55 infectious brain .edema rats in duced by lipid polysaccharide（150 μg,0.15 mL） carotid artery injection were randomly divided into 3 groups：ulinas- tatin group（U group,n=45）;saline group（S group,n=5） ;control group（C group,n=5）. U group were divided into 3 subgroups according to different time points after ulinastatin injecting：6,12,24 h（n= 15）. HE staining, wet and dry weight method,flow cytometry and Gelatinous fiber acidic protein GFAP immunohistoehemistry methods were used respectively to measure the pathological morphology and moisture content of brain tissue,the apsptosis of astroeytes and the activation of astrocytes. Results HE staining showed diffuse edema in all experimental rats;moisture content of brain tissue of group U was lower than that in S group and C group, the difference was statistically sgnifieant（P〈 0.05）, there was no significant difference in C group and S group（P〉 0.05）, all experimental groups showed astro- cytes apoptosis which were most significant at 24 h, U group were significantly lower than C groups and S group, the difference was statistically significant（P〈0.05）. And there exists difference between each subgroups（P〈0.05）. Im- munohistoehemical staining of three groups showed positive expression of GFAP cell. U group showed! enhanced ex pression of GFAP than C group and S group. Concbtsion Ulinastatin showed obvious protective effect of brain in in- fectious brain edema,the possible mechanism may be connected with ulinastatin suppresses astrocyte activation and inhibition of astrocyte apoptosis.

关 键 词：乌司他丁 感染性脑水肿 治疗 机制 

分 类 号：R742.7[医药卫生—神经病学与精神病学]