人牙菌斑及唾液中脂肪酸定性定量研究  

A Comparative Study of Fatty Acids in human Dental Plaque and Whole Saliva

作　　者：周学东[1] 李继遥[1] 戴朝政[2] 张平 

机构地区：[1]华西医科大学口腔医学院,成都610041 [2]中科院成都分院分析测试中心

出　　处：《华西医科大学学报》2000年第2期152-154,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金!39000104

摘　　要：为对人菌斑和唾液的脂肪酸进行定性定量研究，采集10名志愿者的菌斑和唾液经甲酯化预处理，用GC，GC／MS法检测菌斑、唾液中脂肪酸的成份及含量。结果：牙菌斑含有5种直链饱和及3种直链非饱和酸；唾液含有3种直链饱和及3种直链非饱和酸。脂肪酸占菌斑干重为35．15μg／mg，在唾液中含量为13．23μg／ml，C16：0、C18：1是含量最多的脂肪酸。牙菌斑和唾液脂肪酸的成份不同，提示两者的脂肪酸来源和作用可能不同。This study was intended to carry out qualitative and quantitative analyses of fatty acids in plaque and saliva. Samples of plaque and saliva from 10 volunteers were analyzed for fatty acids by gas-chromatography and gas-chromatography / mass spectrometry. The sample preparation procedure allowed specimens from individual volunteers to be analyzed. The results showed the plaque contained C14:0C15:0, C16:0, C16:1,C18:0, C18:1,C18:2,C20:0 acids and the saliva contained C16:0, C18:0, C20:0, C16:1, C18:1,C18:2, acids. The concentration of fattyacids in plaque was 35. 17± 7. 723μg/mg (dry weight) ;and that in saliva was 13. 23μg/ml. Quantitatively, C18:1 and C16:0 acids were the largest fatty acids. These findings imply that the origin and role of the fatty acids in plaque and saliva may be different.

关 键 词：脂肪酸 牙菌斑 唾液 GC GC/MS 龋齿 

分 类 号：R446.5[医药卫生—诊断学]