三磷酸腺苷生物荧光液闪测定法检测活细胞计数  

An Adenosine Triphosphate Bioluminescence Assay for Detecting the number or Living Cells

作　　者：刘珊玲[1] 彭芝兰[1] 王和[1] 楼江燕[1] 何斌[1] 唐茜萍[1] 邱东升 

机构地区：[1]华西医科大学附属第二医院妇产科,成都610041

出　　处：《华西医科大学学报》2000年第2期260-261,268,共3页Journal of West China University of Medical Sciences

基　　金：高等学校博士学科点专项基金

摘　　要：　为建立一种检测活细胞数的方法，作者采用三磷酸腺苷（ATP）生物荧光液闪测定法检测小鼠纤维瘤细胞株L929。ATP标准曲线工作范围为10－9～10－5mol／ml，相关系数r＝0．9963（P＜0．001）；当活细胞数在3×102～106个／ml时与发光计数值有良好的线性关系，r＝0．9922（P＜0．001），变异系数CV＝1％～3％。用ATP生物荧光液闪测定法检测活细胞数灵敏、简便、准确、重复性好，用液闪计数仪即可完成测定，有较大的实用价值。The rnethod for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r=0. 9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r=0. 9922). The detectable cells ranged from 102 to 106 cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.

关 键 词：三磷酸腺苷 生物荧光液闪测定 细胞计数 肿瘤 

分 类 号：R446.1[医药卫生—诊断学]