应用AnnexinV-FITC/PI-FCM定量分析对5-FU诱导卵巢癌细胞系凋亡的研究  

Study of ovarian carcinoma cell line apoptosis induced by 5-FU with Annexin V-FITC/PI-FCM quantitative analysis

作　　者：胡煜[1] 李巨[1] 王喜良 

机构地区：[1]中国人民解放军沈阳军区202医院妇产科,沈阳110003

出　　处：《中国实用妇科与产科杂志》2000年第6期357-358,共2页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的　应用AnnexinV -FITC/PI -FCM定量分析 5 -氟尿嘧啶 (5 -FU)诱导卵巢癌细胞系OVCAR发生的凋亡。方法　将 5 -FU以 0、2 0mg/L、10 0mg/L、2 0 0mg/L的浓度 ,分别处理OVCAR细胞 12、2 4、48小时 ,然后用形态学观察、DNA琼脂糖电泳和AnnexinV -FITC/PI流式细胞技术 (flowcytometric,FCM)检测其诱发凋亡的情况。结果　AnnexinV -FITC -FCM分析见 ,随 5 -FU作用浓度增加、时间延长 ,凋亡细胞比例明显升高 ,差异显著 (P <0 .0 1) ,继发性坏死细胞比例增加 ,差异明显 (P <0 .0 5 )。结论　 5 -FU可以诱发OVCAR细胞发生凋亡 ,应用AnnexinV -FITC/PI -FCM能对细胞凋亡定性的同时测出活细胞、凋亡细胞、继发性坏死细胞和培养操作过程中出现机械损伤细胞的比例 ,与传统方法相比 ,更能直观。Objectve\ Annexin V-FITC/PI-FCM method was used to detect quantitatively the apoptosis of ovarian carcinoma cell line-OVCAR induced by 5-FU.Method\ After the cells had been treated respectively for 12、24、48h by 5-FU of 0、20mg/L、100mg/L、200mg/L,we applied morphology,DNA gel electrophoresis and AnnexinV-FITC/PI flow cytometer method to detect the induced apoptosis condition.Results\ By AnnexinV-FITC/PI-FCM method,we got the following results:the percentage of apoptotic cell increased significantly due to the higher concentration and longer treating duration of 5-Fu (P<0 01);and as well,the percentage of secondary necrotic cell increased too,the increasing was obvious(P<0 05).Conclusion\ 5-FU induces OVCAR cell apoptosis.Using AnnexinV-FITC/PI-FCM can identify the percentage of living cell、apoptotic cell、secondary necrotic cell and mechanical destruction of cell during culture procedure.Compared with the traditional methods,AnnexinV-FITC/PI-FCM method can reflect the relationships of apoptosis with drug concentration and treatment time more accurately.

关 键 词：卵巢癌 5-氟尿嘧啶 ANNEXINV 细胞凋亡 

分 类 号：R73731[医药卫生—肿瘤]