2007-2011年北京市通州区分离单增李斯特菌的生物学及分子流行病学特征  

Biological and molecular epidemiological characteristics of Listeria monocytogenes isolated in Tongzhou,Beijing,2007-2011

作　　者：张兰荣[1] 王艳[2] 张扬[1] 高翔[1] 甄博珺[1] 张冲[1] 邹林[1] 刘晓峰[1] 金东[2] 李伟[2] 王天姝[2] 余波[2] 贺春月[2] 叶长芸[2] 

机构地区：[1]北京市通州区疾病预防控制中心,北京101100 [2]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室

出　　处：《疾病监测》2012年第7期562-564,共3页Disease Surveillance

摘　　要：目的了解2007-2011年北京市通州地区食品及患者分离的单增李斯特菌的生物学和流行病学特征,为李斯特菌病的预防控制提供参考依据。方法对分离的单增李斯特菌进行6个毒力因子(prfA、actA、iap、hly、plcB、inlA)的检测、血清分型和脉冲场凝胶电泳分型。结果 57株单增李斯特菌的6个毒力因子均为阳性,分为5种血清型,主要血清型为1/2a和1/2c型,PFGE分型分为19种带型,主要带型为GX6A16.CN0004。结论通州地区分离的单增李斯特菌全部携带6个主要毒力因子,分子型别呈现多态性。Objective To understand the biological and molecular epidemiological characteristics of Listeria monocytogenes strains isolated in Tongzhou from 2007 to 2010, and provide evidence for the prevention and control of listeriosis. Methods Detection of 6 virulence factors （prfA, actA, iap, hly, plcB, inlA） of L. monocytogenes were conducted using PCR and the strains were characterized by serotyping and PFGE typing. Results The 6 virulence factors were detected in 57 L. monocytogenes strains. The strains belonged to 5 serotypes and 19 PFGE patterns. The predominant serotypes were 1/2a and 1/2c and the major PFGE pattern was GX6A16. CN0004. Conclusion All the L. monocytogenes strains isolated in Tongzhou carried 6 virulence factors and showed polymorphism in molecular type. According to the study result, it is necessary to strengthen the surveillance of L. monocytogenes.

关 键 词：单增李斯特菌 毒力因子 血清型 

分 类 号：R181.3[医药卫生—流行病学]